Glucose- and K(+)-induced acidification in different yeast species
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
10664885
DOI
10.1007/bf02818550
Knihovny.cz E-zdroje
- MeSH
- biologický transport MeSH
- draslík farmakologie MeSH
- erythrosin farmakologie MeSH
- glukosa farmakologie MeSH
- kationty jednomocné farmakologie MeSH
- kyseliny metabolismus MeSH
- protonové ATPasy metabolismus MeSH
- Saccharomycetales účinky léků metabolismus MeSH
- spotřeba kyslíku MeSH
- suloktidil farmakologie MeSH
- vanadáty farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- draslík MeSH
- erythrosin MeSH
- glukosa MeSH
- kationty jednomocné MeSH
- kyseliny MeSH
- protonové ATPasy MeSH
- suloktidil MeSH
- vanadáty MeSH
The process of acidification of the external medium after addition of glucose and subsequently of KCl to a suspension of yeast cells varies substantially from species to species. After glucose it is most pronounced in Saccharomyces cerevisiae and Schizosaccharomyces pombe but is very much lower in Lodderomyces elongisporus, Dipodascus magnusii and Rhodotorula gracilis. Both the buffering capacity and the varied effects of vanadate, suloctidil and erythrosin B indicate that the acidification is by about one-half due to the activity of plasma membrane H(+)-ATPase and by about one-half to the extrusion of acidic metabolites from cells. This is supported by the finding that a respiratory quotient greater than one (in various strains of S. cerevisiae and in S. pombe) is indicative of a greater buffering capacity and overall acidification of the medium. Taking into account the virtually negligible buffering capacity of the medium in the pH range where the effect of K+ is observed, the effect of K+ is generally of a similar magnitude as that of adding glucose. It is clearly dependent on (anaerobic) production of metabolic energy, quite distinct from the dependence of the H(+)-ATPase-caused acidification.
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Folia Microbiol (Praha). 1980;25(4):306-10 PubMed
Cell Mol Biol. 1985;31(3):195-200 PubMed
J Histochem Cytochem. 1992 Jun;40(6):771-9 PubMed
Biochim Biophys Acta. 1981 May 20;643(3):583-92 PubMed
Plant Cell. 1999 Feb;11(2):263-72 PubMed
Folia Microbiol (Praha). 1980;25(4):311-7 PubMed
Biochem Mol Biol Int. 1998 Dec;46(5):973-8 PubMed
Biochem Mol Biol Int. 1998 Feb;44(2):371-80 PubMed
Folia Microbiol (Praha). 1993;38(6):467-72 PubMed
Folia Microbiol (Praha). 1998;43(6):623-5 PubMed
Biochim Biophys Acta. 1991 Dec 12;1071(4):375-91 PubMed
J Biol Chem. 1980 Jun 25;255(12):5735-41 PubMed
Biochem Mol Biol Int. 1994 Aug;33(6):1145-9 PubMed
