Effects of the physiological state of five yeast species on H(+)-ATPase-related processes
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu srovnávací studie, časopisecké články
PubMed
7908655
DOI
10.1007/bf02814397
Knihovny.cz E-zdroje
- MeSH
- aktivní transport MeSH
- diethylstilbestrol farmakologie MeSH
- druhová specificita MeSH
- glukosa farmakologie MeSH
- glutamáty farmakokinetika MeSH
- kinetika MeSH
- koncentrace vodíkových iontů MeSH
- kvasinky účinky léků metabolismus MeSH
- kyselina glutamová MeSH
- membránové potenciály účinky léků MeSH
- protonové ATPasy metabolismus MeSH
- Rhodotorula účinky léků metabolismus MeSH
- Saccharomyces cerevisiae účinky léků metabolismus MeSH
- Saccharomycetales účinky léků metabolismus MeSH
- Schizosaccharomyces účinky léků metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- diethylstilbestrol MeSH
- glukosa MeSH
- glutamáty MeSH
- kyselina glutamová MeSH
- protonové ATPasy MeSH
Effects of starvation and glucose preincubation on membrane potential, ATPase-mediated acidification and glutamic acid transport were studied in yeast species Saccharomyces cerevisiae, Schizosaccharomyces pombe, Dipodascus magnusii, Lodderomyces elongisporus and Rhodotorula gracilis. The membrane potential was highest after preincubation with glucose in all species but L. elongisporus and R. gracilis. In all cases the membranes were depolarized in the presence of 20 mmol/L KCl and hyperpolarized with 50 mumol/L diethylstilbestrol (DES). The extracellular acidification caused by addition of glucose was highest after preincubation with glucose in all cases except in R. gracilis where there was none. In all cases except in R. gracilis addition of KCl caused a marked increase in the acidification rate. Addition of DES with glucose caused a large decrease in rate in S. cerevisiae but had much less effect on the other species. Transport of glutamic acid was clearly increased after pretreatment with glucose in S. cerevisiae, S. pombe and D. magnusii (mainly due to enhanced synthesis of the carrier) but actually decreased in R. gracilis and L. elongisporus. Addition of DES had an inhibitory effect in all species but much more pronounced in S. cerevisiae and S. pombe than in others. In general, both the acidification and the transport of glutamate were enhanced after preincubation with glucose but much more so in the semianaerobic species, such as S. cerevisiae, than in the strict aerobes (R. gracilis) where the effect was occasionally negative. There was no relationship between the ATPase-mediated acidification and the membrane potential.
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