Dependence of phosphate transport in yeast of glycolytic substrates
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
7033082
DOI
10.1007/bf02927330
Knihovny.cz E-zdroje
- MeSH
- fosfáty metabolismus MeSH
- glukosa farmakologie MeSH
- Saccharomyces cerevisiae metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- fosfáty MeSH
- glukosa MeSH
Preincubation of baker's yeast (wild strain, respiration-deficient mutant and a low-phosphorus culture) with glucose, trehalose, and other metabolic sugars increases the subsequent uptake of inorganic phosphate 3-5 times. The Kt is reduced by the preincubation from 3.5 to 1.6 mM. The process involves primarily the production of glycolytic energy sources (suppression by iodoacetamide, no effect of antimycin or dicyclohexylcarbodiimide, negligible effect of ethanol, or respiratory mutation). The low-phosphorus yeast takes up phosphate anions about 1-20 times faster than the high-phosphorus (normal) culture. The stimulation is also accompanied by some (apparently nonessential) protein synthesis and has a halftime of 35 min; its decay has a t0.5 of 12 min but affects only less than one-half of the stimulated capacity.
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