Dependence of phosphate transport in yeast of glycolytic substrates
Language English Country United States Media print
Document type Journal Article
PubMed
7033082
DOI
10.1007/bf02927330
Knihovny.cz E-resources
- MeSH
- Phosphates metabolism MeSH
- Glucose pharmacology MeSH
- Saccharomyces cerevisiae metabolism MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Phosphates MeSH
- Glucose MeSH
Preincubation of baker's yeast (wild strain, respiration-deficient mutant and a low-phosphorus culture) with glucose, trehalose, and other metabolic sugars increases the subsequent uptake of inorganic phosphate 3-5 times. The Kt is reduced by the preincubation from 3.5 to 1.6 mM. The process involves primarily the production of glycolytic energy sources (suppression by iodoacetamide, no effect of antimycin or dicyclohexylcarbodiimide, negligible effect of ethanol, or respiratory mutation). The low-phosphorus yeast takes up phosphate anions about 1-20 times faster than the high-phosphorus (normal) culture. The stimulation is also accompanied by some (apparently nonessential) protein synthesis and has a halftime of 35 min; its decay has a t0.5 of 12 min but affects only less than one-half of the stimulated capacity.
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