Effects of the physiological state of five yeast species on H(+)-ATPase-related processes
Language English Country United States Media print
Document type Comparative Study, Journal Article
PubMed
7908655
DOI
10.1007/bf02814397
Knihovny.cz E-resources
- MeSH
- Biological Transport, Active MeSH
- Diethylstilbestrol pharmacology MeSH
- Species Specificity MeSH
- Glucose pharmacology MeSH
- Glutamates pharmacokinetics MeSH
- Kinetics MeSH
- Hydrogen-Ion Concentration MeSH
- Yeasts drug effects metabolism MeSH
- Glutamic Acid MeSH
- Membrane Potentials drug effects MeSH
- Proton-Translocating ATPases metabolism MeSH
- Rhodotorula drug effects metabolism MeSH
- Saccharomyces cerevisiae drug effects metabolism MeSH
- Saccharomycetales drug effects metabolism MeSH
- Schizosaccharomyces drug effects metabolism MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- Diethylstilbestrol MeSH
- Glucose MeSH
- Glutamates MeSH
- Glutamic Acid MeSH
- Proton-Translocating ATPases MeSH
Effects of starvation and glucose preincubation on membrane potential, ATPase-mediated acidification and glutamic acid transport were studied in yeast species Saccharomyces cerevisiae, Schizosaccharomyces pombe, Dipodascus magnusii, Lodderomyces elongisporus and Rhodotorula gracilis. The membrane potential was highest after preincubation with glucose in all species but L. elongisporus and R. gracilis. In all cases the membranes were depolarized in the presence of 20 mmol/L KCl and hyperpolarized with 50 mumol/L diethylstilbestrol (DES). The extracellular acidification caused by addition of glucose was highest after preincubation with glucose in all cases except in R. gracilis where there was none. In all cases except in R. gracilis addition of KCl caused a marked increase in the acidification rate. Addition of DES with glucose caused a large decrease in rate in S. cerevisiae but had much less effect on the other species. Transport of glutamic acid was clearly increased after pretreatment with glucose in S. cerevisiae, S. pombe and D. magnusii (mainly due to enhanced synthesis of the carrier) but actually decreased in R. gracilis and L. elongisporus. Addition of DES had an inhibitory effect in all species but much more pronounced in S. cerevisiae and S. pombe than in others. In general, both the acidification and the transport of glutamate were enhanced after preincubation with glucose but much more so in the semianaerobic species, such as S. cerevisiae, than in the strict aerobes (R. gracilis) where the effect was occasionally negative. There was no relationship between the ATPase-mediated acidification and the membrane potential.
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