In a worldwide majority of sheep breeds an excessive susceptibility to scrapie associated with the PrP gene alleles coding for valine (V; at the 136 codon) and glutamine (Q; at the 171 codon) (e.g., VRQ/VRQ, VRQ/ARQ, or ARQ/ARQ) was demonstrated. Particularly the PrPVRQ allele is closely associated with the high-risk development of the disease; the PrPARQ allele can also fulfill this function but under certain limited conditions. Polymorphism in the PrP gene sequences (conclusively related to the increased susceptibility of sheep to scrapie) of improved Valachian sheep from two Slovak regions, Orava and Spis, was determined. Examination of 735 sheep showed that ARR/ARQ was the most frequent genotype (45.2%). High-risk genotypes were determined in 32.4% of sheep (ARQ/ARQ 19.3, ARR/VRQ 9.0, ARR/VRQ 3.5, VRQ/VRQ 0.3, ARR/VRR 0.3). Low-risk genotypes were found in 67.7% of sheep (ARR/ARQ 45.2, ARR/ARR 10.9, ARR/AHQ 5.7, ARQ/ARQ 4.9, AHQ/AHQ 0.7, ARR/AHR 0.3). Despite the geographically distant flocks of improved Valachian sheep investigated no difference in the occurrence of individual PrP genotypes was observed.

Institute of Microbiology and Immunology University of Veterinary Medicine 041 81 Kosice Slovakia

Zobrazit více v PubMed

Eur J Epidemiol. 1991 Sep;7(5):520-3 PubMed

J Gen Virol. 1999 Sep;80 ( Pt 9):2527-34 PubMed

J Infect Dis. 1998 Oct;178(4):949-53 PubMed

APMIS. 2002 Jan;110(1):33-43 PubMed

Arch Virol. 1999;144(3):431-45 PubMed

Proc Natl Acad Sci U S A. 1997 May 13;94(10):4931-6 PubMed

J Comp Pathol. 2002 Jan;126(1):17-29 PubMed

J Gen Virol. 1993 Jun;74 ( Pt 6):1025-31 PubMed

Arch Virol. 1999;144(4):829-34 PubMed

Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13363-83 PubMed

EMBO J. 1985 Sep;4(9):2309-12 PubMed

J Gen Virol. 1994 May;75 ( Pt 5):989-95 PubMed

Vet Rec. 1998 Jun 6;142(23):623-5 PubMed

J Gen Virol. 1991 Oct;72 ( Pt 10):2411-7 PubMed

Genes Dev. 1994 Apr 15;8(8):959-69 PubMed

Folia Microbiol (Praha). 2000;45(6):475-83 PubMed

Vet Rec. 2000 Mar 18;146(12):335-8 PubMed

J Gen Virol. 1996 Oct;77 ( Pt 10):2669-73 PubMed

Nature. 1997 Mar 13;386(6621):137 PubMed

J Gen Virol. 1995 Aug;76 ( Pt 8):2097-101 PubMed

J Gen Virol. 1999 Apr;80 ( Pt 4):1073-7 PubMed

Ciba Found Symp. 1988;135:63-83 PubMed

Mamm Genome. 1993;4(8):463-4 PubMed

Arch Virol. 2001 Oct;146(10):2029-37 PubMed

Science. 1982 Apr 9;216(4542):136-44 PubMed

J Gen Virol. 1998 Aug;79 ( Pt 8):2079-82 PubMed

J Gen Virol. 1995 Oct;76 ( Pt 10):2577-81 PubMed

J Gen Virol. 1995 Mar;76 ( Pt 3):509-17 PubMed

Genetic diversity of the prion protein gene (PRNP) coding sequence in Czech sheep and evaluation of the national breeding programme for resistance to scrapie in the Czech Republic