Genetic diversity of the prion protein gene (PRNP) coding sequence in Czech sheep and evaluation of the national breeding programme for resistance to scrapie in the Czech Republic

. 2017 Feb ; 58 (1) : 111-121. [epub] 20160523

Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/pmid27215403
Odkazy

PubMed 27215403
DOI 10.1007/s13353-016-0354-5
PII: 10.1007/s13353-016-0354-5
Knihovny.cz E-zdroje

Of 34 breeds kept in the Czech Republic 45,604 sheep were genotyped for codons 136, 154 and 171 in the prion protein gene (PRNP) during the years 2006-2014. In this cohort, haplotypes ARR, ARQ, ARH, AHQ, VRQ, AHR and ARK were detected. The haplotype AF141RQ associated with susceptibility to atypical scrapie was observed in nine out of 30 breeds analysed for this purpose. In addition, six rare nonsynonymous substitutions producing haplotypes AT137RQ, AN138RQ, AG151RQ, AH151RQ, ARL168Q and ARQE175 were identified in various breeds. Due to their low frequencies, these polymorphisms are of no potential importance for the breeding programme. With regard to their genetic particularity, Sumavka, Valachian and Cameroon breeds were screened for additional polymorphisms. Further haplotypes, AR143RQ and AS146RQ, were found in Sumavka and Cameroon, and in Valachian sheep, respectively. Frequencies of the ARR (resistance-associated), VRQ (susceptibility-associated) haplotypes, and of the most resistant ARR/ARR genotype calculated for sheep born in the years 2001-2003 and 2011-2013 documented effects of the 10 year-lasting national breeding programme. The total frequency of ARR doubled from 36.8 to 75.8 %, while the frequency of VRQ decreased from 4 to 0.7 %. The total frequency of the ARR/ARR genotype increased from 17.7 to 59 %. These data show that the national scrapie resistance breeding programme has had an important desirable effect on haplotype and genotype frequencies of PRNP in Czech sheep.

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Prev Vet Med. 2014 Nov 1;117(1):242-50 PubMed

Biochem Biophys Res Commun. 2005 Oct 21;336(2):544-53 PubMed

J Virol. 2007 Jul;81(13):7306-9 PubMed

Virol J. 2011 Dec 24;8:559 PubMed

Vet Res. 2009 May-Jun;40(3):19 PubMed

J Dairy Sci. 2013 Sep;96(9):6079-83 PubMed

J Gen Virol. 2005 Jan;86(Pt 1):231-5 PubMed

Biochem Genet. 2012 Apr;50(3-4):277-84 PubMed

Anim Genet. 2009 Dec;40(6):982-5 PubMed

Vet Res. 2008 Jul-Aug;39(4):19 PubMed

Folia Microbiol (Praha). 2003;48(2):269-76 PubMed

Vet J. 2011 Feb;187(2):245-50 PubMed

Tijdschr Diergeneeskd. 2011 Feb 1;136(2):84-93 PubMed

Trop Anim Health Prod. 2012 Oct;44(7):1469-72 PubMed

Vet Rec. 2007 Oct 13;161(15):524-5 PubMed

Bioinform Biol Insights. 2015 Sep 24;9(Suppl 4):1-10 PubMed

Vet Res. 2011 Feb 15;42:30 PubMed

J Vet Sci. 2013;14(1):45-51 PubMed

BMC Vet Res. 2009 Jan 21;5:3 PubMed

Genet Sel Evol. 2010 May 18;42:14 PubMed

Science. 2007 Nov 9;318(5852):930-6 PubMed

J Gen Virol. 2002 Mar;83(Pt 3):713-21 PubMed

Vet Rec. 2014 May 24;174(21):530 PubMed

Vet Res. 2008 Jul-Aug;39(4):25 PubMed

Curr Opin Genet Dev. 2013 Jun;23(3):345-51 PubMed

J Gen Virol. 2004 Jul;85(Pt 7):2103-10 PubMed

J Gen Virol. 2006 May;87(Pt 5):1395-402 PubMed

Vet Rec. 1993 Oct 2;133(14):339-41 PubMed

Vet Rec. 2003 Aug 16;153(7):202-8 PubMed

Vet Res. 2008 Jul-Aug;39(4):28 PubMed

BMC Vet Res. 2010 May 14;6:25 PubMed

J Gen Virol. 2004 Sep;85(Pt 9):2735-40 PubMed

Front Immunol. 2015 Sep 02;6:450 PubMed

Animal. 2009 Mar;3(3):336-46 PubMed

J Gen Virol. 2006 Dec;87(Pt 12):3741-5 PubMed

BMC Vet Res. 2010 Feb 07;6:9 PubMed

Anim Genet. 2011 Aug;42(4):406-14 PubMed

Vet Res. 2008 Jul-Aug;39(4):30 PubMed

J Gen Virol. 1995 Mar;76 ( Pt 3):509-17 PubMed

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