Purcopia, a Ty1-copia truncated retroelement in the genome of Claviceps purpurea
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12879745
DOI
10.1007/bf02931365
Knihovny.cz E-resources
- MeSH
- Claviceps genetics MeSH
- DNA, Fungal chemistry genetics MeSH
- Phylogeny MeSH
- Genome, Fungal MeSH
- Cloning, Molecular MeSH
- Molecular Sequence Data MeSH
- Polymerase Chain Reaction MeSH
- Retroelements genetics MeSH
- Amino Acid Sequence MeSH
- Base Sequence MeSH
- Sequence Alignment MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Fungal MeSH
- Retroelements MeSH
Truncated copy of reverse transcriptase of Ty1/copia retroelement (Purcopia) was found as part of the species-specific RAPD 257(540) marker of Claviceps purpurea. A region of 94 bp with 78.9% identity to an unannotated region of the genomic clone of the rice blast fungus Pyricularia grisea (accession no. AQ162050) was found at the 5' end of the pseudogene. Comparison with database sequences revealed that Purcopia is close to the plant retroelements represented by Tto1, Ta1-3 and Bare-1, whereas the other fungal elements of the Ty1/copia type grouped with Hopscotch elements. Restriction patterns obtained by hybridization of the labeled marker to HindIII digested genomic DNA of various C. purpurea isolates contained multiple bands. The banding was individual and did not yield any species- or population-specific fragments or patterns.
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