A novel family of miniature transposable elements, named Zaba, was identified in pea (Pisum sativum) and subsequently also in other legume species using computer analysis of their DNA sequences. Zaba elements are 141-190 bp long, generate 10-bp target site duplications, and their terminal inverted repeats make up most of the sequence. Zaba elements thus resemble class 3 foldback transposons. The elements are only moderately repetitive in pea (tens to hundreds copies per haploid genome), but they are present in up to thousands of copies in the genomes of several Medicago and Vicia species. More detailed analysis of the elements from pea, including isolation of new sequences from a genomic library, revealed that a fraction of these elements are truncated, and that their last transposition probably did not occur recently. A search for Zaba sequences in EST databases showed that at least some elements are transcribed, most probably due to their association with genic regions.

Laboratory of Molecular Cytogenetics Institute of Plant Molecular Biology Branisovská 31 37005 Ceské Budejovice Czech Republic

Zobrazit více v PubMed

Plant Mol Biol. 1996 Dec;32(6):1221-6 PubMed

Cytometry. 2002 Jan 1;47(1):1-7 PubMed

Plant J. 1999 May;18(4):431-41 PubMed

Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8 PubMed

Plant J. 1999 Sep;19(5):591-7 PubMed

Plant Mol Biol. 2000 Jan;42(1):251-69 PubMed

Trends Genet. 1992 Mar;8(3):103-8 PubMed

Nat Rev Genet. 2002 May;3(5):329-41 PubMed

Plant Cell. 1992 Oct;4(10):1283-94 PubMed

Mol Gen Genet. 2000 Jul;263(6):898-907 PubMed

Genome Res. 2000 Jul;10(7):982-90 PubMed

Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PubMed

Plant Mol Biol. 1997 Jul;34(5):831-5 PubMed

J Mol Evol. 1999 Jan;48(1):13-21 PubMed

Plant Mol Biol. 1990 Aug;15(2):295-306 PubMed

Gene. 1995 Dec 29;167(1-2):GC1-10 PubMed

Genome. 2001 Aug;44(4):716-28 PubMed

Plant Cell. 1994 Jun;6(6):907-16 PubMed

Proc Natl Acad Sci U S A. 2000 Feb 1;97(3):1160-5 PubMed

Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1460-5 PubMed

Nucleic Acids Res. 1997 Sep 1;25(17):3389-402 PubMed

A chromosome-scale reference genome of grasspea (Lathyrus sativus)

Accumulation of retrotransposons contributes to W chromosome differentiation in the willow beauty Peribatodes rhomboidaria (Lepidoptera: Geometridae)

Repetitive DNA in the pea (Pisum sativum L.) genome: comprehensive characterization using 454 sequencing and comparison to soybean and Medicago truncatula