Macrococcus brunensis sp. nov., Macrococcus hajekii sp. nov. and Macrococcus lamae sp. nov., from the skin of llamas
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
13130064
DOI
10.1099/ijs.0.02683-0
Knihovny.cz E-resources
- MeSH
- RNA, Bacterial genetics MeSH
- DNA, Bacterial chemistry genetics MeSH
- Phenotype MeSH
- Phylogeny MeSH
- Skin microbiology MeSH
- Camelids, New World microbiology MeSH
- Fatty Acids analysis MeSH
- Molecular Sequence Data MeSH
- DNA, Ribosomal genetics MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Base Sequence MeSH
- Staphylococcaceae classification genetics isolation & purification metabolism MeSH
- Base Composition MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- RNA, Bacterial MeSH
- DNA, Bacterial MeSH
- Fatty Acids MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal, 16S MeSH
Eight strains of Gram-positive, catalase- and oxidase-positive cocci were isolated from the skin of llamas (Lama glama L.) and characterized using a polyphasic approach. These strains were assigned to the genus Macrococcus on the basis of their phenotypic properties (resistance to bacitracin and sensitivity to furazolidone) and DNA base content (40-42 mol% G+C). Phylogenetic analysis based on 16S rDNA confirmed that the strains are members of the genus MACROCOCCUS: They differed from all hitherto described macrococcal species in their production of phosphatase and reduction of nitrate (most strains) and the inability to produce acid from glycerol or to grow in 7.5 % NaCl. Ribotyping (EcoRI), macrorestriction analysis (XbaI) and fatty acid methyl ester analysis divided the strains from llamas into three stable clusters. Moreover, ribotyping differentiated the strains analysed not only from previously described macrococcal species but also from oxidase-positive staphylococci. DNA-DNA hybridization confirmed that the three clusters represent separate genomic groups (similarity values<54 %). All the results showed that the strains represent three novel species, for which the names Macrococcus hajekii sp. nov. (type strain CCM 4809(T)=LMG 21711(T)), Macrococcus brunensis sp. nov. (type strain CCM 4811(T)=LMG 21712(T)) and Macrococcus lamae sp. nov. (type strain CCM 4815(T)=LMG 21713(T)) are proposed.
References provided by Crossref.org
Staphylococcus ratti sp. nov. Isolated from a Lab Rat
GENBANK
AY119685, AY119686, AY119687
