Telomeres, besides their main role in the protection and maintenance of chromosome ends, have several other vital functions in the cell cycle. We studied their role in the achiasmatic meiosis of female Lepidoptera, insects with holokinetic chromosomes. By fluorescence in-situ hybridization (FISH) with the insect telomeric probe, (TTAGG)n, we mapped the distribution of telomeric and interstitial telomeric sequences (ITS) in female meiotic chromosomes of two species, Orgyia antiqua with a reduced chromosome number (2n = 28) and Ephestia kuehniella mutants, possessing a radiation-induced chromosome fusion in the genome (2n = 59). In addition to the strong typical telomeric signals, O. antiqua displayed weaker hybridization signals in interstitial sites of pachytene bivalents. The observed ITS most probably reflect remnants of chromosomal rearrangements and support the hypothesis that the Orgyia karyotype had arisen by multiple fusions of ancestral chromosomes. On the other hand, the absence of ITS in the chromosome fusion of Ephestia indicated the loss of telomeres before the two original chromosomes fused. When the telomeric probe was amplified by enzymatic reaction with tyramid, the number of ITS observed increased in Orgyia, and a few ITS were also observed in several chromosomes of Ephestia but not in the fused chromosome. This suggests that the genomes of both species also contain ITS other than those originating from chromosome fusions. The analysis of female meiotic prophase I revealed non-homologous associations of postpachytene bivalents mediated by telomeric DNA, which were not observed in the pachytene stage. Surprisingly, in early postpachytene nuclei the telomeric associations also involved ITS, whereas later postpachytene nuclei displayed chains of bivalents interconnected only by true telomeres. This finding favours a hypothesis that telomeric associations between bivalents play a role in chromosome segregation in the achiasmatic meiosis of female Lepidoptera.

Department of Genetics Institute of Entomology ASCR Branisovská 31 CZ 370 05 Ceské Budĕjovice Czech Republic

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Annu Rev Genet. 1989;23:579-604 PubMed

Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9051-5 PubMed

Chromosoma. 1977 Apr 19;60(3):205-21 PubMed

Histol Histopathol. 1999 Apr;14(2):517-24 PubMed

Chromosome Res. 1998 Sep;6(6):455-62 PubMed

Chromosome Res. 2002;10(6):445-53 PubMed

Genes Dev. 1998 Aug 15;12(16):2574-86 PubMed

Chromosoma. 1990 Apr;99(1):3-10 PubMed

Nucleic Acids Res. 1991 Sep 11;19(17):4780 PubMed

Chromosoma. 1998 May;107(2):136-40 PubMed

Plant Cell. 1999 Apr;11(4):523-34 PubMed

Chromosome Res. 2002;10(4):279-86 PubMed

Trends Genet. 1989 Oct;5(10):326-31 PubMed

Cytogenet Cell Genet. 1999;86(3-4):288-95 PubMed

Chromosome Res. 2002;10(7):527-34 PubMed

Curr Biol. 1994 Aug 1;4(8):724-7 PubMed

Cytogenet Cell Genet. 1996;72(4):310-5 PubMed

Cytogenet Cell Genet. 1993;63(3):156-9 PubMed

Mutat Res. 1998 Aug 3;404(1-2):199-204 PubMed

Chromosoma. 2000;109(1-2):3-9 PubMed

Science. 2001 May 11;292(5519):1075-6 PubMed

Nature. 1991 Oct 3;353(6343):451-4 PubMed

Chromosome Res. 2002;10(4):297-304 PubMed

Mol Cell Biol. 1993 Mar;13(3):1424-32 PubMed

Nature. 1991 Apr 18;350(6319):569-73 PubMed

Cell. 1994 Jul 1;77(7):957-62 PubMed

EMBO J. 1997 Jan 2;16(1):193-202 PubMed

Chromosoma. 1999 Aug;108(4):209-19 PubMed

Genetica. 2000;108(2):197-202 PubMed

Chromosome Res. 1998 Dec;6(8):643-8 PubMed

Nature. 1990 Aug 30;346(6287):868-71 PubMed

Eur J Hum Genet. 2002 Feb;10(2):107-12 PubMed

Nat Rev Mol Cell Biol. 2001 Aug;2(8):621-7 PubMed

J Cell Sci. 1999 Mar;112 ( Pt 5):651-8 PubMed

Chromosoma. 2001 May;110(2):75-82 PubMed

EMBO J. 1992 Feb;11(2):717-24 PubMed

Chromosome Res. 2002;10(5):399-406 PubMed

Biochem Biophys Res Commun. 2001 Jan 19;280(2):471-5 PubMed

Bioessays. 2001 Jun;23(6):526-33 PubMed

Chromosome Res. 2002;10(8):655-67 PubMed

Environ Mol Mutagen. 1993;22(4):198-207 PubMed

Chromosoma. 2002 Mar;111(1):45-52 PubMed

EMBO J. 2000 Jul 17;19(14):3831-40 PubMed

Chromosome Res. 2000;8(7):585-91 PubMed

Chromosome Res. 1999;7(6):449-60 PubMed

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