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Effects of a novel anti-exospore monoclonal antibody on microsporidial development in vitro

Sak, B
Author Sak, B Institute of Parasitology, Academy of Sciences of the Czech Republic and Faculty of Biological Sciences, University of South Bohemia, Branisovská 31, 370 05 Ceské Budejovice, Czech Republic. casio@paru.cas.cz
Saková, K
Author Saková, K
Ditrich, O
Author Ditrich, O

Parasitology research. 2004 Jan ; 92 (1) : 74-80. [epub] 20031111

Parasitol Res
ISSN 0932-0113
Source

Language English Country Germany Media print-electronic

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link https://www.medvik.cz/link/pmid14610668

PubMed 14610668
DOI 10.1007/s00436-003-0988-1
Knihovny.cz E-resources

MeSH
Antigens, Protozoan immunology MeSH
Chlorocebus aethiops MeSH
Encephalitozoon cuniculi immunology physiology MeSH
Interferon-gamma metabolism MeSH
Cells, Cultured MeSH
Macrophages MeSH
Antibodies, Monoclonal immunology MeSH
Mice, Inbred BALB C MeSH
Mice MeSH
Antibodies, Protozoan immunology MeSH
Sensitivity and Specificity MeSH
Antibody Specificity MeSH
Spores, Protozoan immunology physiology MeSH
Vero Cells MeSH
Animals MeSH
Check Tag
Mice MeSH
Female MeSH
Animals MeSH
Publication type
Journal Article MeSH
Research Support, Non-U.S. Gov't MeSH
Names of Substances
Antigens, Protozoan MeSH
Interferon-gamma MeSH
Antibodies, Monoclonal MeSH
Antibodies, Protozoan MeSH

The possible role of humoral antibodies in the immune response to microsporidiosis was studied using a novel anti-exospore monoclonal antibody (MAb) P5/H1 which recognised Encephalitozoon cuniculi. The effect of the P5/H1 MAb on microsporidial growth in vitro resulted in a reduction of the numbers of E. cuniculi spores in a Vero E6 cell-line culture. This reduction in the number of infected cells and the decrease of intracellular spores in infected cells was found when MAb P5/H1 was present in cultures, compared to cultures with an irrelevant isotype control MAb. Moreover, the presence of P5/H1 MAb increased the number of phagocytosed spores in macrophage cultures, and increased the activation of macrophages measured by nitrite oxide production. These results suggest a possible partial role of specific humoral antibodies in the protection against E. cuniculi infection.

Institute of Parasitology Academy of Sciences of the Czech Republic and Faculty of Biological Sciences University of South Bohemia Branisovská 31 370 05 Ceské Budejovice Czech Republic

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