Efficiency of chlorocatechol metabolism in natural and constructed chlorobenzoate and chlorobiphenyl degraders
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
14962122
DOI
10.1111/j.1365-2672.2004.02075.x
PII: 2075
Knihovny.cz E-resources
- MeSH
- Biodegradation, Environmental MeSH
- Burkholderia cepacia genetics metabolism MeSH
- Chlorobenzoates metabolism MeSH
- Chlorides analysis MeSH
- DNA, Bacterial MeSH
- Electrochemistry MeSH
- Genetic Engineering MeSH
- Catechols metabolism MeSH
- Polychlorinated Biphenyls metabolism MeSH
- Pseudomonas fluorescens genetics metabolism MeSH
- Spectrophotometry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Chlorobenzoates MeSH
- Chlorides MeSH
- DNA, Bacterial MeSH
- Catechols MeSH
- Polychlorinated Biphenyls MeSH
AIMS: A possibility for the complementation of both ortho- and meta-cleavage pathway for chlorocatechols in one strain and its impact on degradation of chlorobenzoates accumulated during degradation of polychlorinated biphenyls was investigated. METHODS AND RESULTS: Genes responsible for ortho-cleavage of chlorocatechols were subcloned into two biphenyl degraders and the activities of chlorocatechol dioxygenases responsible for ortho- and meta-cleavage in these hybrid strains were monitored spectrophotometrically and also electrochemically by ion-selective electrode. CONCLUSIONS: While strain Pseudomonas fluorescens S12/C apparently gained metabolic advantage from this gene manipulation, strain Burkholderia cepacia P166/C did not express better degradation features in comparison with the parental strain. SIGNIFICANCE AND IMPACT OF THE STUDY: This approach has the potential to enhance chlorocatechol metabolism in selected biphenyl degraders.
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General and molecular microbiology and microbial genetics in the IM CAS
