Efficiency of chlorocatechol metabolism in natural and constructed chlorobenzoate and chlorobiphenyl degraders
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
14962122
DOI
10.1111/j.1365-2672.2004.02075.x
PII: 2075
Knihovny.cz E-zdroje
- MeSH
- biodegradace MeSH
- Burkholderia cepacia genetika metabolismus MeSH
- chlorbenzoáty metabolismus MeSH
- chloridy analýza MeSH
- DNA bakterií MeSH
- elektrochemie MeSH
- genetické inženýrství MeSH
- katecholy metabolismus MeSH
- polychlorované bifenyly metabolismus MeSH
- Pseudomonas fluorescens genetika metabolismus MeSH
- spektrofotometrie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- chlorbenzoáty MeSH
- chloridy MeSH
- DNA bakterií MeSH
- katecholy MeSH
- polychlorované bifenyly MeSH
AIMS: A possibility for the complementation of both ortho- and meta-cleavage pathway for chlorocatechols in one strain and its impact on degradation of chlorobenzoates accumulated during degradation of polychlorinated biphenyls was investigated. METHODS AND RESULTS: Genes responsible for ortho-cleavage of chlorocatechols were subcloned into two biphenyl degraders and the activities of chlorocatechol dioxygenases responsible for ortho- and meta-cleavage in these hybrid strains were monitored spectrophotometrically and also electrochemically by ion-selective electrode. CONCLUSIONS: While strain Pseudomonas fluorescens S12/C apparently gained metabolic advantage from this gene manipulation, strain Burkholderia cepacia P166/C did not express better degradation features in comparison with the parental strain. SIGNIFICANCE AND IMPACT OF THE STUDY: This approach has the potential to enhance chlorocatechol metabolism in selected biphenyl degraders.
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