The W303 genetic background affects the isw2 delta mutant phenotype in Saccharomyces cerevisiae
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15058186
DOI
10.1007/bf02931508
Knihovny.cz E-resources
- MeSH
- Adenosine Triphosphatases genetics MeSH
- Cell Wall MeSH
- Gene Deletion MeSH
- Phenotype MeSH
- Genotype MeSH
- Haploidy MeSH
- Saccharomyces cerevisiae Proteins genetics MeSH
- Saccharomyces cerevisiae cytology genetics MeSH
- Transcription Factors genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adenosine Triphosphatases MeSH
- ISWI protein MeSH Browser
- Saccharomyces cerevisiae Proteins MeSH
- Transcription Factors MeSH
We performed detailed phenotypic analysis of the isw2 delta strains of the W303 genetic background and compared its results with those obtained previously in BY-derived genetic background. Shmoolike morphology was observed in the isw2 delta strain of alpha-mating type of the BY strains, but not in its W303-derived counterpart. On the other hand, derepression of a-specific genes in the isw2 delta (MAT alpha) strain was observed in both genetic backgrounds, although to a different extent. Unlike in BY-derived strain hyperactivation of the Ras2/cAMP pathway reduced invasiveness of the isw2 delta strain (MAT alpha) of the W303 background. Sensitivity to Calcofluor White indicating a cell wall-integrity defect was significantly increased in the isw2 delta strains of the W303 background in contrast to BY-derived strains. Our data indicate that the effects of the isw2 deletion strongly depend on the background in which the deletion, is made.
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