GATC-specific restriction--modification systems in ruminal bacteria
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15227796
DOI
10.1007/bf02931400
Knihovny.cz E-resources
- MeSH
- Rumen microbiology MeSH
- Bacteria enzymology isolation & purification MeSH
- DNA Restriction-Modification Enzymes metabolism MeSH
- DNA, Viral metabolism MeSH
- Electrophoresis, Agar Gel MeSH
- Megasphaera enzymology isolation & purification MeSH
- Deoxyribonucleases, Type II Site-Specific metabolism MeSH
- Substrate Specificity MeSH
- Veillonellaceae enzymology isolation & purification MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA Restriction-Modification Enzymes MeSH
- DNA, Viral MeSH
- GATC-specific type II deoxyribonucleases MeSH Browser
- Deoxyribonucleases, Type II Site-Specific MeSH
The GATC-specific restriction and modification activities were analyzed in 11 major bacterial representatives of ruminal microflora. Modification phenotype was observed in 13 out of 40 ruminal strains. MboI isoschizomeric restriction endonucleases were detected in 10 bacterial strains tested; three strains lacked any detectable corresponding endonuclease activity. The only examined strain of Mitsuokella multi-acida was found to possess a different type of endonuclease activity. This is the first report on restriction activity in ruminal treponemes M. multiacida and Megasphaera elsdenii.
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