Differentiation of HL-60 myeloid leukemia cells induced by all-trans retinoic acid is enhanced in combination with caffeic acid
Language English Country Greece Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15254783
Knihovny.cz E-resources
- MeSH
- Leukemia, Promyelocytic, Acute drug therapy MeSH
- Antigens, Neoplasm biosynthesis MeSH
- Coloring Agents pharmacology MeSH
- Cell Differentiation MeSH
- Time Factors MeSH
- Antigens, CD MeSH
- Models, Chemical MeSH
- GPI-Linked Proteins MeSH
- HL-60 Cells MeSH
- Immunophenotyping MeSH
- Caffeic Acids administration & dosage chemistry MeSH
- Humans MeSH
- Cell Adhesion Molecules biosynthesis MeSH
- Cell Proliferation MeSH
- Antineoplastic Combined Chemotherapy Protocols therapeutic use MeSH
- Flow Cytometry MeSH
- Drug Synergism * MeSH
- Tetrazolium Salts pharmacology MeSH
- Thiazoles pharmacology MeSH
- Tretinoin administration & dosage MeSH
- Dose-Response Relationship, Drug MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, Neoplasm MeSH
- Coloring Agents MeSH
- caffeic acid MeSH Browser
- Antigens, CD MeSH
- CEACAM8 protein, human MeSH Browser
- GPI-Linked Proteins MeSH
- Caffeic Acids MeSH
- Cell Adhesion Molecules MeSH
- Tetrazolium Salts MeSH
- Thiazoles MeSH
- thiazolyl blue MeSH Browser
- Tretinoin MeSH
We investigated a possible enhancement of all-trans retinoic acid (ATRA)-induced differentiation of HL-60 human myeloid leukemia cells by caffeic acid (CA), a widely distributed plant phenolic compound. Our results showed that CA, in the concentration of 13 or 52 micro M, had no or minimal influence on cell differentiation, whereas the differentiating activity of ATRA was potentiated by CA treatment. We proved, using flow cytometric detection of the CD66b surface molecule, a synergistic effect of CA: at day 10, 18.3% of CD66b-positive cells were detected after treatment with ATRA only, and 33% when CA and ATRA were combined together. NBT-assay confirmed that this additive effect of CA on ATRA-induced differentiation. Proliferating activity as assessed by MTT-assay was generally not affected by CA at given concentrations. However, cell proliferation was significantly reduced by 52 micro M CA at 96-h intervals. This effect was markedly enhanced when CA, at both concentrations, and ATRA were combined. The possibility to enhance the differentiation potential of ATRA by CA may improve outcomes in the therapy of acute promyelocytic leukemia.
Why Differentiation Therapy Sometimes Fails: Molecular Mechanisms of Resistance to Retinoids
