Expression of Rgmc, the murine ortholog of hemojuvelin gene, is modulated by development and inflammation, but not by iron status or erythropoietin
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15315977
DOI
10.1182/blood-2004-06-2422
PII: S0006-4971(20)49080-9
Knihovny.cz E-resources
- MeSH
- Erythropoietin pharmacology MeSH
- GPI-Linked Proteins MeSH
- Hepcidins MeSH
- Homeostasis MeSH
- Liver embryology growth & development MeSH
- Antimicrobial Cationic Peptides genetics MeSH
- RNA, Messenger genetics MeSH
- Mice MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Muscle Proteins genetics MeSH
- Gene Expression Regulation, Developmental drug effects genetics MeSH
- Inflammation physiopathology MeSH
- Iron metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Erythropoietin MeSH
- GPI-Linked Proteins MeSH
- HAMP protein, human MeSH Browser
- Hamp protein, mouse MeSH Browser
- Hepcidins MeSH
- Antimicrobial Cationic Peptides MeSH
- RNA, Messenger MeSH
- Rgmc protein, mouse MeSH Browser
- Muscle Proteins MeSH
- Iron MeSH
Mutations of hepcidin (HAMP) and hemo-juvelin (HJV) genes have been recently demonstrated to result in juvenile hemochromatosis. Expression of HAMP is regulated by iron status or infection, whereas regulation of HJV is yet unknown. Using quantitative real-time polymerase chain reaction, we compared expression of Hamp and Rgmc (the murine ortholog of HJV) in livers of mice treated with iron, erythropoietin, or lipopolysaccharide (LPS), as well as during fetal and postnatal development. Iron overload increased Hamp expression without effect on Rgmc mRNA. Erythropoietin decreased Hamp mRNA, but Rgmc expression was unchanged. Hamp mRNA level decreased after birth by 4 orders of magnitude, without significant changes in Rgmc expression. Administration of LPS elevated Hamp mRNA levels, while markedly decreasing hepatic Rgmc mRNA levels (to approximately 5% after 6 hours). The responses of Hamp and Rgmc were quite different and suggested that human HJV expression could be modulated by inflammation.
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