Reinnervation of the rat musculocutaneous nerve stump after its direct reconnection with the C5 spinal cord segment by the nerve graft following avulsion of the ventral spinal roots: a comparison of intrathecal administration of brain-derived neurotrophic factor and Cerebrolysin
Language English Country Germany Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Amino Acids administration & dosage pharmacology MeSH
- Axons physiology MeSH
- Choline O-Acetyltransferase metabolism MeSH
- Behavior, Animal drug effects MeSH
- Dextrans MeSH
- Fluorescent Dyes MeSH
- Immunohistochemistry MeSH
- Muscle, Skeletal innervation MeSH
- Rats MeSH
- Skin innervation MeSH
- Spinal Cord physiology MeSH
- Spinal Nerve Roots physiology MeSH
- Spinal Nerves physiology transplantation MeSH
- Motor Neurons drug effects physiology MeSH
- Brain-Derived Neurotrophic Factor administration & dosage pharmacology MeSH
- Nerve Fibers, Myelinated physiology MeSH
- Musculocutaneous Nerve cytology physiology MeSH
- Neuroprotective Agents administration & dosage pharmacology MeSH
- Rats, Wistar MeSH
- Nerve Regeneration physiology MeSH
- Rhodamines MeSH
- Injections, Spinal MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Amino Acids MeSH
- cerebrolysin MeSH Browser
- Choline O-Acetyltransferase MeSH
- Dextrans MeSH
- Fluorescent Dyes MeSH
- Fluoro-Ruby MeSH Browser
- Brain-Derived Neurotrophic Factor MeSH
- Neuroprotective Agents MeSH
- Rhodamines MeSH
Experimental model based on the C5 ventral root avulsion was used to evaluate the efficacy of brain-derived neurotrophic factor (BDNF) and Cerebrolysin treatment on motor neuron maintenance and survival resulted in the functional reinnervation of the nerve stump. In contrast to vehicle, BDNF treatment reduced the loss and atrophy of motor neurons and enhanced the regrowth axon sprouts into the distal stump of musculocutaneous nerve. However, the axon diameter of the myelinated fibers was smaller than those of control rats. The morphometric results were related to a low score in behavioral test similar to vehicle-treated rats. Cerebrolysin treatment greatly protected the motor neurons against cell death. Moreover, morphometric features of myelinated axons were better than those of rats treated with vehicle or BDNF. The mean score of grooming test suggested better results of the functional motor reinnervation than after BDNF administration. The majority of rescued motor neurons regenerating their axons through nerve graft in both BDNF- and Cerebrolysin-treated rats expressed choline acetyltransferase immunostaining. The results demonstrate that BDNF has more modest effects in preventing the death of motor neurons and functional recovery of injured motor nerve after root avulsion than Cerebrolysin.
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J Neural Transm Suppl. 1998;53:289-98 PubMed
J Neurobiol. 1994 Jul;25(7):759-66 PubMed
J Neurosci. 1995 Feb;15(2):1044-56 PubMed
J Neurosci Methods. 1995 May;58(1-2):151-5 PubMed
Ann Anat. 2003 Jun;185(3):233-8 PubMed
Neurorehabil Neural Repair. 2000;14(4):319-30 PubMed
Prog Brain Res. 1988;78:219-24 PubMed
Brain Res. 1983 Jan 17;259(1):41-56 PubMed
Exp Neurol. 1996 Oct;141(2):190-200 PubMed
Brain Res Brain Res Rev. 2002 Oct;40(1-3):292-300 PubMed
Neuron. 1996 Aug;17(2):195-7 PubMed
J Neurosci Methods. 1993 Mar;46(3):203-8 PubMed
Annu Rev Neurosci. 1996;19:463-89 PubMed
J Neurosci Methods. 1995 May;58(1-2):29-37 PubMed
Exp Neurol. 1993 Apr;120(2):153-9 PubMed
J Comp Neurol. 1994 Apr 1;342(1):35-44 PubMed
Proc Natl Acad Sci U S A. 1995 Oct 10;92(21):9771-5 PubMed
Neuroscience. 2003;122(2):381-9 PubMed
Pharmacopsychiatry. 1994 Jan;27(1):32-40 PubMed
Curr Opin Neurobiol. 1994 Oct;4(5):668-79 PubMed
Science. 1994 Aug 19;265(5175):1107-10 PubMed
J Neural Transm Suppl. 1998;53:351-61 PubMed
Histol Histopathol. 1992 Apr;7(2):213-21 PubMed
Neurosci Lett. 1995 Nov 10;200(1):45-8 PubMed
Rev Neurol. 1998 Apr;26(152):551-4 PubMed
J Neurobiol. 1993 Aug;24(8):1065-79 PubMed
Nature. 1992 Dec 24-31;360(6406):755-7 PubMed
Brain Res. 1989 Mar 20;482(2):205-18 PubMed
Ann Anat. 2000 Mar;182(2):123-31 PubMed
Exp Neurol. 1997 Apr;144(2):273-86 PubMed
No To Shinkei. 1993 Apr;45(4):325-31 PubMed
Neuroreport. 1994 Feb 24;5(6):693-6 PubMed
Acta Physiol Scand. 1986 Dec;128(4):645-6 PubMed
Lancet. 1995 Nov 18;346(8986):1323-5 PubMed
J Comp Neurol. 1994 Apr 1;342(1):45-56 PubMed
J Comp Neurol. 1992 Jul 22;321(4):576-90 PubMed
J Neurosci. 2000 Sep 15;20(18):6962-7 PubMed
