Experimental model based on the C5 ventral root avulsion was used to evaluate the efficacy of brain-derived neurotrophic factor (BDNF) and Cerebrolysin treatment on motor neuron maintenance and survival resulted in the functional reinnervation of the nerve stump. In contrast to vehicle, BDNF treatment reduced the loss and atrophy of motor neurons and enhanced the regrowth axon sprouts into the distal stump of musculocutaneous nerve. However, the axon diameter of the myelinated fibers was smaller than those of control rats. The morphometric results were related to a low score in behavioral test similar to vehicle-treated rats. Cerebrolysin treatment greatly protected the motor neurons against cell death. Moreover, morphometric features of myelinated axons were better than those of rats treated with vehicle or BDNF. The mean score of grooming test suggested better results of the functional motor reinnervation than after BDNF administration. The majority of rescued motor neurons regenerating their axons through nerve graft in both BDNF- and Cerebrolysin-treated rats expressed choline acetyltransferase immunostaining. The results demonstrate that BDNF has more modest effects in preventing the death of motor neurons and functional recovery of injured motor nerve after root avulsion than Cerebrolysin.

Department of Neurosurgery 3rd Faculty of Medicine Charles University Prague Czech Republic

Zobrazit více v PubMed

J Neural Transm Suppl. 1998;53:289-98 PubMed

J Neurobiol. 1994 Jul;25(7):759-66 PubMed

J Neurosci. 1995 Feb;15(2):1044-56 PubMed

J Neurosci Methods. 1995 May;58(1-2):151-5 PubMed

Ann Anat. 2003 Jun;185(3):233-8 PubMed

Neurorehabil Neural Repair. 2000;14(4):319-30 PubMed

Prog Brain Res. 1988;78:219-24 PubMed

Brain Res. 1983 Jan 17;259(1):41-56 PubMed

Exp Neurol. 1996 Oct;141(2):190-200 PubMed

Brain Res Brain Res Rev. 2002 Oct;40(1-3):292-300 PubMed

Neuron. 1996 Aug;17(2):195-7 PubMed

J Neurosci Methods. 1993 Mar;46(3):203-8 PubMed

Annu Rev Neurosci. 1996;19:463-89 PubMed

J Neurosci Methods. 1995 May;58(1-2):29-37 PubMed

Exp Neurol. 1993 Apr;120(2):153-9 PubMed

J Comp Neurol. 1994 Apr 1;342(1):35-44 PubMed

Proc Natl Acad Sci U S A. 1995 Oct 10;92(21):9771-5 PubMed

Neuroscience. 2003;122(2):381-9 PubMed

Pharmacopsychiatry. 1994 Jan;27(1):32-40 PubMed

Curr Opin Neurobiol. 1994 Oct;4(5):668-79 PubMed

Science. 1994 Aug 19;265(5175):1107-10 PubMed

J Neural Transm Suppl. 1998;53:351-61 PubMed

Histol Histopathol. 1992 Apr;7(2):213-21 PubMed

Neurosci Lett. 1995 Nov 10;200(1):45-8 PubMed

Rev Neurol. 1998 Apr;26(152):551-4 PubMed

J Neurobiol. 1993 Aug;24(8):1065-79 PubMed

Nature. 1992 Dec 24-31;360(6406):755-7 PubMed

Brain Res. 1989 Mar 20;482(2):205-18 PubMed

Ann Anat. 2000 Mar;182(2):123-31 PubMed

Exp Neurol. 1997 Apr;144(2):273-86 PubMed

No To Shinkei. 1993 Apr;45(4):325-31 PubMed

Neuroreport. 1994 Feb 24;5(6):693-6 PubMed

Acta Physiol Scand. 1986 Dec;128(4):645-6 PubMed

Lancet. 1995 Nov 18;346(8986):1323-5 PubMed

J Comp Neurol. 1994 Apr 1;342(1):45-56 PubMed

J Comp Neurol. 1992 Jul 22;321(4):576-90 PubMed

J Neurosci. 2000 Sep 15;20(18):6962-7 PubMed

Ciliary neurotrophic factor promotes motor reinnervation of the musculocutaneous nerve in an experimental model of end-to-side neurorrhaphy