Comparison between mitochondrial and chloroplast DNA variation in the native range of Silene vulgaris
Language English Country Great Britain, England Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
PubMed
15367108
DOI
10.1111/j.1365-294x.2004.02278.x
PII: MEC2278
Knihovny.cz E-resources
- MeSH
- DNA, Chloroplast genetics MeSH
- Genetic Variation * MeSH
- Haplotypes genetics MeSH
- Crosses, Genetic MeSH
- DNA, Mitochondrial genetics MeSH
- Molecular Sequence Data MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Reproduction genetics MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Silene genetics MeSH
- Blotting, Southern MeSH
- Geography MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Comparative Study MeSH
- Geographicals
- Europe MeSH
- Names of Substances
- DNA, Chloroplast MeSH
- DNA, Mitochondrial MeSH
A detailed survey of mitochondrial and chloroplast diversity in eight populations of Silene vulgaris from Central Europe was conducted for comparison with previously published data on diversity from S. vulgaris populations in the introduced range. Mitochondrial DNA (mtDNA) variation around the coxI gene was assessed with Southern blotting/restriction fragment length polymorphism methods. Chloroplast variation was assessed by sequencing the intergenic spacer separating the trnH and psbA genes. Thirty mtDNA haplotypes and 24 chloroplast DNA (cpDNA) haplotypes were found within 86 individuals. The overall genetic diversity h (0.941 for mitochondrial, and 0.893 for chloroplast markers) and within-population diversity were higher than reported in previous population studies of S. vulgaris in the USA and Europe. The frequency of private alleles was surprisingly high - more than 90% for both kinds of markers. Most of our populations were large and located in relatively undisturbed meadows, whereas surveys in Virginia consisted of smaller roadside populations. The slow rate of population turnover in European populations is discussed as a factor responsible for the relatively high diversity of S. vulgaris in undisturbed areas of its native range. Association between mtDNA and cpDNA haplotypes was also demonstrated. Finally, gender and mtDNA haplotype were associated in the Alps populations, where females were very rare.
References provided by Crossref.org
Non-coding RNA may be associated with cytoplasmic male sterility in Silene vulgaris
Mosaic origins of a complex chimeric mitochondrial gene in Silene vulgaris