Production of secreted aspartate proteinases was determined in a set of 646 isolates of Candida and non-Candida yeast species collected from 465 patients of the University Hospital in Olomouc (Czechia) in the period 1995-2002, and Candida samples obtained from 64 healthy volunteers using solid media developed for this purpose. Using random amplified polymorphic DNA analysis (RAPD) 79 Candida isolates from blood were analyzed to show potential relationships between clustering of the fingerprints and extracellular proteolytic activity of these strains. C. albicans, C. tropicalis and C. parapsilosis possess always proteolytic activity while non-Candida species did not display any proteolysis. A tight relationship between fingerprints and extracellular proteolysis in the Candida isolates was not shown. A remarkable consistency between fingerprint clusters and proteolysis occurred in a subset of C. parapsilosis samples. Suboptimal pH of the growth medium was shown to facilitate the investigation of potential co-incidence of genotypic and phenotypic traits.

Institute of Microbiology Faculty of Medicine Palacký University Olomouc Czechia

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J Gen Microbiol. 1993 Sep;139(9):2179-84 PubMed

Am J Med. 1996 Jun;100(6):617-23 PubMed

Clin Microbiol Rev. 1996 Oct;9(4):499-511 PubMed

J Clin Microbiol. 1999 Sep;37(9):2817-28 PubMed

J Clin Microbiol. 2001 Sep;39(9):3360-1 PubMed

Folia Microbiol (Praha). 2001;46(4):359-60 PubMed

Folia Microbiol (Praha). 2002;47(2):179-81 PubMed

Rev Iberoam Micol. 2002 Sep;19(3):139-43 PubMed

Mycoses. 2003 Apr;46(3-4):90-5 PubMed

Trends Microbiol. 2001 Dec;9(12):591-6 PubMed

Compr Ther. 1995 Nov;21(11):653-7 PubMed

Trends Microbiol. 1998 Dec;6(12):468-70 PubMed

Antimicrob Agents Chemother. 2001 Apr;45(4):1174-83 PubMed

Folia Microbiol (Praha). 2002;47(2):105-12 PubMed

Braz J Infect Dis. 2000 Aug;4(4):168-72 PubMed

J Clin Microbiol. 2003 Feb;41(2):712-6 PubMed

Dent Update. 2001 Apr;28(3):132-9 PubMed

Arch Intern Med. 1995 Jun 12;155(11):1177-84 PubMed

Folia Microbiol (Praha). 2002;47(6):727-31 PubMed

Folia Microbiol (Praha). 2003;48(2):173-6 PubMed

Folia Microbiol (Praha). 2002;47(2):182-4 PubMed

Folia Microbiol (Praha). 2002;47(3):302-4 PubMed

Curr Med Chem. 2001 Jul;8(8):941-8 PubMed

Antimicrob Agents Chemother. 1998 Oct;42(10):2645-9 PubMed

J Med Microbiol. 1999 Aug;48(8):721-30 PubMed

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