Distribution of secreted aspartyl proteinases using a polymerase chain reaction assay with SAP specific primers in Candida albicans isolates
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
16475500
DOI
10.1007/bf02931422
Knihovny.cz E-zdroje
- MeSH
- aspartátové endopeptidasy analýza genetika MeSH
- Candida albicans enzymologie genetika izolace a purifikace MeSH
- DNA fungální analýza MeSH
- fungální proteiny analýza genetika MeSH
- geny hub MeSH
- izoenzymy antagonisté a inhibitory genetika MeSH
- kandidóza mikrobiologie MeSH
- krev mikrobiologie MeSH
- lidé MeSH
- polymerázová řetězová reakce metody MeSH
- vagina mikrobiologie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- aspartátové endopeptidasy MeSH
- DNA fungální MeSH
- fungální proteiny MeSH
- izoenzymy MeSH
- SAP1 protein, Candida albicans MeSH Prohlížeč
- SAP2 protein, Candida MeSH Prohlížeč
- SAP3 protein, Candida albicans MeSH Prohlížeč
- SAP4 protein, Candida albicans MeSH Prohlížeč
- SAP5 protein, Candida albicans MeSH Prohlížeč
Secreted aspartyl proteinase (Sap) distribution among different C. albicans isolates was determined using SAP-specific primers in polymerase chain reaction (PCR) assay. SAP1, SAP2, and SAP3 were detected in 13 of 40 (32.5%), SAP4 in 38/40 (95%), SAP5 were detected in 30/40 (75%), SAP6 in 23/40 (57.5%) of C. albicans strains isolated from blood cultures. SAP1-SAP3 were detected in 37 of 40 (92.5%), SAP4 were detected in 3/40 (7.5%), SAP5 in 3/40 (7.5%), SAP6 in 5/40 (12.5%) of C. albicans strains isolated from vaginal swab cultures. Sap1, Sap2 and Sap3 isoenzymes were found to be related to the vaginopathic potential of C. albicans; Sap4, Sap5 and Sap6 isoenzymes were found to be correlated with systemic infections.
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