The collection wild-type strain of Candida albicans was used to obtain auxotrophic and colony-morphology mutants by 'nitrosoguanidine' treatment. Intraspecific protoplast fusion induced by Ca(2+)-poly(ethyleneglycol) was carried out in various pairings between the auxotrophic strain producing smooth colonies and containing blastospores and the colony-morphology mutants containing a mixture of blastospores and pseudohyphae or only hyphae. Hybrids exhibiting full or partial complementation were obtained when mutants producing smooth colonies and colony-morphology variants of different origins were fused. The mutation responsible for the colony-morphology character (if various types of colony morphomutants were crossed) proved to be recessive or semidominant. Representative hybrids exhibited elevated DNA contents as measured by flow cytometry. To illustrate various cell types, and especially the intermediate one (never observed in natural isolates), a preparation method was further developed for scanning electron microscopic studies.

Department of General and Environmental Microbiology University of Pécs 7601 Pécs Hungary

Zobrazit více v PubMed

Clin Microbiol Rev. 1992 Apr;5(2):183-203 PubMed

Yeast. 1996 Dec;12(16):1677-702 PubMed

Med Mycol. 2000;38 Suppl 1:79-86 PubMed

J Bacteriol. 1971 Apr;106(1):276-80 PubMed

J Gen Microbiol. 1982 Jan;128(1):123-8 PubMed

J Med Microbiol. 1994 Jun;40(6):416-23 PubMed

J Bacteriol. 1981 Jun;146(3):833-40 PubMed

Folia Microbiol (Praha). 2002;47(2):179-81 PubMed

EMBO J. 1997 Apr 15;16(8):1982-91 PubMed

Crit Rev Microbiol. 1985;12(3):223-70 PubMed

Arch Microbiol. 1977 Nov 18;115(2):143-9 PubMed

J Bacteriol. 1973 Jul;115(1):341-8 PubMed

Acta Microbiol Acad Sci Hung. 1978;25(3):209-12 PubMed

J Immunol Methods. 2001 Nov 1;257(1-2):185-202 PubMed

Folia Microbiol (Praha). 2001;46(5):443-6 PubMed

J Bacteriol. 1987 Jan;169(1):189-97 PubMed

J Infect. 1998 Jan;36(1):57-62 PubMed

J Bacteriol. 1991 Oct;173(20):6586-96 PubMed

J Med Microbiol. 1999 Feb;48(2):167-72 PubMed

Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13223-8 PubMed

Folia Microbiol (Praha). 2002;47(2):105-12 PubMed

J Bacteriol. 1986 Jan;165(1):61-5 PubMed

Microbiol Rev. 1990 Sep;54(3):226-41 PubMed

Nature. 1977 Aug 11;268(5620):524-5 PubMed

J Bacteriol. 1970 Nov;104(2):902-9 PubMed

J Gen Microbiol. 1988 Jun;134(6):1587-95 PubMed

Crit Rev Microbiol. 1987;15(1):79-85 PubMed

Folia Microbiol (Praha). 2002;47(6):727-31 PubMed

J Bacteriol. 1993 Nov;175(22):7189-99 PubMed

Trends Microbiol. 2001 Jul;9(7):327-35 PubMed

Curr Genet. 1990 Apr;17(4):293-6 PubMed

Folia Microbiol (Praha). 2001;46(5):453-7 PubMed

Acta Biol Hung. 2001;52(2-3):289-98 PubMed

Mol Gen Genet. 1985;200(1):162-8 PubMed

Science. 1997 Jul 4;277(5322):105-9 PubMed

Arch Microbiol. 1981 Mar;129(1):1-8 PubMed

Detection of Candida albicans by culture, serology and PCR in clinical specimens from patients with ulcerative colitis: re-evaluation of an old hypothesis with a new perspective

Distribution of secreted aspartyl proteinases using a polymerase chain reaction assay with SAP specific primers in Candida albicans isolates

Characterization of Candida albicans colony morphological mutants and their hybrids by means of RAPD-PCR, isoenzyme analysis and pathogenicity analysis