Detection of Candida albicans by culture, serology and PCR in clinical specimens from patients with ulcerative colitis: re-evaluation of an old hypothesis with a new perspective
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
16295666
DOI
10.1007/bf02931575
Knihovny.cz E-zdroje
- MeSH
- biologické modely MeSH
- Candida albicans genetika imunologie izolace a purifikace patogenita MeSH
- DNA fungální analýza genetika MeSH
- feces mikrobiologie MeSH
- imunoglobulin G krev MeSH
- imunoglobulin M krev MeSH
- lidé MeSH
- polymerázová řetězová reakce MeSH
- protilátky fungální krev MeSH
- sekvence nukleotidů MeSH
- střevní sliznice mikrobiologie MeSH
- studie případů a kontrol MeSH
- ulcerózní kolitida etiologie imunologie mikrobiologie patologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- DNA fungální MeSH
- imunoglobulin G MeSH
- imunoglobulin M MeSH
- protilátky fungální MeSH
The relationship between inflammatory bowel disease and microorganisms was evaluated. The presence of Candida albicans-specific IgM and IgG antibodies in serum samples and the presence of C. albicans in stool and colonal mucosa samples of the patients did not exhibit any significant difference between 21 patients in active stage and 15 patients in remission of ulcerative colitis (UC) (compared with 19 control patients). The invasion of yeast cells to the colonal mucosa was demonstrated by detecting C. albicans DNA using specific PCon1, PCon2, and PspA2 primers in PCR assay. Eighteen of 36 patients (50%) were found to be DNA positive while in 19 controls only 4 (21%) were found to be positive. The presence of DNA in the association of the positive serological reactivity is suggested as an important diagnostic marker of UC.
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