Detection of Candida albicans by culture, serology and PCR in clinical specimens from patients with ulcerative colitis: re-evaluation of an old hypothesis with a new perspective
Language English Country United States Media print
Document type Journal Article
PubMed
16295666
DOI
10.1007/bf02931575
Knihovny.cz E-resources
- MeSH
- Models, Biological MeSH
- Candida albicans genetics immunology isolation & purification pathogenicity MeSH
- DNA, Fungal analysis genetics MeSH
- Feces microbiology MeSH
- Immunoglobulin G blood MeSH
- Immunoglobulin M blood MeSH
- Humans MeSH
- Polymerase Chain Reaction MeSH
- Antibodies, Fungal blood MeSH
- Base Sequence MeSH
- Intestinal Mucosa microbiology MeSH
- Case-Control Studies MeSH
- Colitis, Ulcerative etiology immunology microbiology pathology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA, Fungal MeSH
- Immunoglobulin G MeSH
- Immunoglobulin M MeSH
- Antibodies, Fungal MeSH
The relationship between inflammatory bowel disease and microorganisms was evaluated. The presence of Candida albicans-specific IgM and IgG antibodies in serum samples and the presence of C. albicans in stool and colonal mucosa samples of the patients did not exhibit any significant difference between 21 patients in active stage and 15 patients in remission of ulcerative colitis (UC) (compared with 19 control patients). The invasion of yeast cells to the colonal mucosa was demonstrated by detecting C. albicans DNA using specific PCon1, PCon2, and PspA2 primers in PCR assay. Eighteen of 36 patients (50%) were found to be DNA positive while in 19 controls only 4 (21%) were found to be positive. The presence of DNA in the association of the positive serological reactivity is suggested as an important diagnostic marker of UC.
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Infect Immun. 2004 Jan;72(1):209-18 PubMed
J Clin Microbiol. 1997 Jun;35(6):1353-60 PubMed
Gut. 1991 Jan;32(1):55-60 PubMed
J Clin Microbiol. 2003 Nov;41(11):4986-90 PubMed
Clin Exp Immunol. 1979 Jan;35(1):147-8 PubMed
Comp Biochem Physiol B Biochem Mol Biol. 2000 May;126(1):109-20 PubMed
Folia Microbiol (Praha). 2004;49(4):491-6 PubMed
Clin Diagn Lab Immunol. 1996 Mar;3(2):219-26 PubMed
Hepatogastroenterology. 1989 Aug;36(4):182-4 PubMed
J Clin Microbiol. 2004 Mar;42(3):1129-35 PubMed
Mycoses. 2001;44(5):151-6 PubMed
Gut. 1990 May;31(5):536-8 PubMed
Gastroenterology. 1987 Feb;92(2):479-85 PubMed
J Microbiol Methods. 2004 Feb;56(2):201-11 PubMed
BMJ. 1988 Oct 29;297(6656):1105-6 PubMed
J Clin Microbiol. 1998 May;36(5):1169-75 PubMed
Scand J Gastroenterol. 2001 Feb;36(2):196-201 PubMed
Diagn Microbiol Infect Dis. 2003 Jun;46(2):103-8 PubMed
Clin Microbiol Rev. 2002 Jan;15(1):79-94 PubMed
Folia Microbiol (Praha). 2003;48(4):555-8 PubMed
Folia Microbiol (Praha). 2004;49(5):527-33 PubMed
Folia Microbiol (Praha). 2004;49(4):484-90 PubMed
Folia Microbiol (Praha). 2003;48(2):173-6 PubMed
J Med Vet Mycol. 1988 Jun;26(3):173-85 PubMed
J Med Microbiol. 1980 Feb;13(1):103-10 PubMed
Mycoses. 1991 Jul-Aug;34(7-8):293-6 PubMed
Folia Microbiol (Praha). 2003;48(2):203-9 PubMed
FEMS Microbiol Immunol. 1992 Apr;4(4):219-24 PubMed
Diagn Microbiol Infect Dis. 2002 Jan;42(1):39-42 PubMed
J Clin Microbiol. 2004 Feb;42(2):867-70 PubMed
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