Cryopreservation of isolated mouse germinal vesicles
Language English Country Japan Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15599110
DOI
10.1262/jrd.16071
PII: JST.JSTAGE/jrd/16071
Knihovny.cz E-resources
- MeSH
- Fertilization in Vitro * MeSH
- Cryopreservation instrumentation methods MeSH
- Mice, Inbred ICR MeSH
- Mice MeSH
- Oocytes cytology MeSH
- Swine MeSH
- Cell Survival MeSH
- Zona Pellucida MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The storage of unfertilized oocytes, either immature, maturing or mature, is still unsatisfactory. Here we describe an approach in which germinal vesicles isolated as karyoplasts from immature oocytes are vitrified by open the pulled straws (OPS) method in evacuated porcine zonae pellucidae. After thawing, their survival was almost absolute. Moreover, when thawed GV-karyoplasts were fused to immature oocyte cytoplasts the maturation of reconstructed cells resulted in the production of secondary oocytes--metaphase II.
References provided by Crossref.org
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