Cryopreservation of isolated mouse germinal vesicles
Jazyk angličtina Země Japonsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
15599110
DOI
10.1262/jrd.16071
PII: JST.JSTAGE/jrd/16071
Knihovny.cz E-zdroje
- MeSH
- fertilizace in vitro * MeSH
- kryoprezervace přístrojové vybavení metody MeSH
- myši inbrední ICR MeSH
- myši MeSH
- oocyty cytologie MeSH
- prasata MeSH
- viabilita buněk MeSH
- zona pellucida MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The storage of unfertilized oocytes, either immature, maturing or mature, is still unsatisfactory. Here we describe an approach in which germinal vesicles isolated as karyoplasts from immature oocytes are vitrified by open the pulled straws (OPS) method in evacuated porcine zonae pellucidae. After thawing, their survival was almost absolute. Moreover, when thawed GV-karyoplasts were fused to immature oocyte cytoplasts the maturation of reconstructed cells resulted in the production of secondary oocytes--metaphase II.
Citace poskytuje Crossref.org
Improving the Quality of Oocytes with the Help of Nucleolotransfer Therapy
