Polyunsaturated fatty acids sensitize human colon adenocarcinoma HT-29 cells to death receptor-mediated apoptosis
Jazyk angličtina Země Irsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
15639338
DOI
10.1016/j.canlet.2004.07.038
PII: S0304-3835(04)00621-4
Knihovny.cz E-zdroje
- MeSH
- adenokarcinom patologie MeSH
- apoptóza účinky léků MeSH
- kolagen typ XI metabolismus MeSH
- kyseliny arachidonové farmakologie MeSH
- kyseliny dokosahexaenové farmakologie MeSH
- lidé MeSH
- mitochondrie MeSH
- nádory tračníku patologie MeSH
- peroxidace lipidů MeSH
- poly(ADP-ribosa)polymerasa 1 MeSH
- poly(ADP-ribosa)polymerasy MeSH
- proliferace buněk MeSH
- protilátky farmakologie MeSH
- reaktivní formy kyslíku MeSH
- TNF-alfa farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- CH-11 anti-fas antibody, human MeSH Prohlížeč
- COL11A2 protein, human MeSH Prohlížeč
- kolagen typ XI MeSH
- kyseliny arachidonové MeSH
- kyseliny dokosahexaenové MeSH
- PARP1 protein, human MeSH Prohlížeč
- poly(ADP-ribosa)polymerasa 1 MeSH
- poly(ADP-ribosa)polymerasy MeSH
- protilátky MeSH
- reaktivní formy kyslíku MeSH
- TNF-alfa MeSH
The proliferative and apoptotic response to TNF-alpha and anti-Fas antibody (CH-11) in human colon adenocarcinoma HT-29 cells was modulated by pretreatment with arachidonic (AA, 20:4, n-6) or docosahexaenoic (DHA, 22:6, n-3) fatty acids, which alone increased reactive oxygen species production and lipid peroxidation, and decreased the S-phase of the cell cycle. The higher amount of floating cells, subG0/G1 population and apoptotic cells detected in pre-treated cells was potentiated by cycloheximide. The effects of CH-11 were associated with activation of caspase-8, -9, and -3, cleavage of poly(ADP-ribose)polymerase-PARP, and decreased mitochondrial membrane potential (MMP), but these parameters were not significantly changed after PUFA pretreatment.
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