Polyunsaturated fatty acids sensitize human colon adenocarcinoma HT-29 cells to death receptor-mediated apoptosis
Language English Country Ireland Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15639338
DOI
10.1016/j.canlet.2004.07.038
PII: S0304-3835(04)00621-4
Knihovny.cz E-resources
- MeSH
- Adenocarcinoma pathology MeSH
- Apoptosis drug effects MeSH
- Collagen Type XI metabolism MeSH
- Arachidonic Acids pharmacology MeSH
- Docosahexaenoic Acids pharmacology MeSH
- Humans MeSH
- Mitochondria MeSH
- Colonic Neoplasms pathology MeSH
- Lipid Peroxidation MeSH
- Poly (ADP-Ribose) Polymerase-1 MeSH
- Poly(ADP-ribose) Polymerases MeSH
- Cell Proliferation MeSH
- Antibodies pharmacology MeSH
- Reactive Oxygen Species MeSH
- Tumor Necrosis Factor-alpha pharmacology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- CH-11 anti-fas antibody, human MeSH Browser
- COL11A2 protein, human MeSH Browser
- Collagen Type XI MeSH
- Arachidonic Acids MeSH
- Docosahexaenoic Acids MeSH
- PARP1 protein, human MeSH Browser
- Poly (ADP-Ribose) Polymerase-1 MeSH
- Poly(ADP-ribose) Polymerases MeSH
- Antibodies MeSH
- Reactive Oxygen Species MeSH
- Tumor Necrosis Factor-alpha MeSH
The proliferative and apoptotic response to TNF-alpha and anti-Fas antibody (CH-11) in human colon adenocarcinoma HT-29 cells was modulated by pretreatment with arachidonic (AA, 20:4, n-6) or docosahexaenoic (DHA, 22:6, n-3) fatty acids, which alone increased reactive oxygen species production and lipid peroxidation, and decreased the S-phase of the cell cycle. The higher amount of floating cells, subG0/G1 population and apoptotic cells detected in pre-treated cells was potentiated by cycloheximide. The effects of CH-11 were associated with activation of caspase-8, -9, and -3, cleavage of poly(ADP-ribose)polymerase-PARP, and decreased mitochondrial membrane potential (MMP), but these parameters were not significantly changed after PUFA pretreatment.
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