Construction of two new vectors for transformation of laboratory, natural and industrial Saccharomyces cerevisiae strains to trifluoroleucine and G418 resistance
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
15702541
DOI
10.1007/bf02931529
Knihovny.cz E-zdroje
- MeSH
- DNA fungální genetika MeSH
- druhová specificita MeSH
- fungální léková rezistence genetika MeSH
- genetické vektory MeSH
- gentamiciny farmakologie MeSH
- geny hub MeSH
- leucin analogy a deriváty farmakologie MeSH
- mutageneze cílená MeSH
- plazmidy genetika MeSH
- Saccharomyces cerevisiae účinky léků genetika MeSH
- sekvence nukleotidů MeSH
- transformace genetická MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 5',5',5'-trifluoroleucine MeSH Prohlížeč
- antibiotic G 418 MeSH Prohlížeč
- DNA fungální MeSH
- gentamiciny MeSH
- leucin MeSH
Two new plasmids, pEC3 and pECkan, were constructed and their use in yeast transformation described. Both plasmids are derivative of the pRS416 vector, in which the URA3 auxotrophic marker was replaced by the LEU4* gene (pEC3) or the kanMX4 gene (pECkan). pEC3 and pECkan plasmids transformed natural and commercial Saccharomyces cerevisiae strains to 5,5,5-trifluoro-DL-leucine and G418 (aminoglycoside related to gentamicin) resistance, respectively, with efficiency ranging from 10(-5) to 10(-7) transformants per number of viable cells. pEC3 transformed the Leu- laboratory strain, carrying the mutations leu4 leu9, to leucine prototrophy with efficiency of approximately 10(-4).
Zobrazit více v PubMed
Nucleic Acids Res. 1998 Aug 1;26(15):3611-3 PubMed
Yeast. 2002 Jan 15;19(1):17-28 PubMed
J Biotechnol. 2001 Dec 14;92(1):27-35 PubMed
Appl Microbiol Biotechnol. 2001 Sep;56(5-6):577-88 PubMed
Yeast. 1994 Dec;10(13):1793-808 PubMed
Genetics. 1989 May;122(1):19-27 PubMed
Mol Gen Genet. 1999 Feb;261(1):152-60 PubMed
FEMS Microbiol Lett. 1996 Apr 1;137(2-3):165-8 PubMed
Yeast. 1997 May;13(6):551-9 PubMed
Yeast. 1990 Nov-Dec;6(6):521-34 PubMed
FEMS Microbiol Lett. 1999 Nov 15;180(2):229-33 PubMed
FEBS Lett. 1998 Mar 20;425(1):117-22 PubMed
J Biol Chem. 1995 Mar 10;270(10):5270-5 PubMed
Methods Enzymol. 1991;194:319-29 PubMed
Yeast. 2000 Apr;16(6):539-45 PubMed
Yeast. 1998 Jan 30;14(2):115-32 PubMed
Folia Microbiol (Praha). 2003;48(4):496-500 PubMed
