Cross-resistance to strobilurin fungicides in mitochondrial and nuclear mutants of Saccharomyces cerevisiae
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
14533481
DOI
10.1007/bf02931331
Knihovny.cz E-resources
- MeSH
- ATP-Binding Cassette Transporters genetics MeSH
- Acrylates pharmacology MeSH
- Antifungal Agents pharmacology MeSH
- Cell Nucleus genetics MeSH
- DNA-Binding Proteins genetics MeSH
- Phenylacetates pharmacology MeSH
- Fungal Proteins genetics MeSH
- Genes, Fungal MeSH
- Methacrylates MeSH
- Microbial Sensitivity Tests MeSH
- Mitochondria genetics MeSH
- Drug Resistance, Multiple, Fungal genetics MeSH
- Mutation MeSH
- Fatty Acids, Unsaturated pharmacology MeSH
- Fungicides, Industrial pharmacology MeSH
- Pyrimidines pharmacology MeSH
- Saccharomyces cerevisiae Proteins genetics MeSH
- Saccharomyces cerevisiae drug effects genetics MeSH
- Strobilurins MeSH
- Trans-Activators genetics MeSH
- Transcription Factors genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- ATP-Binding Cassette Transporters MeSH
- Acrylates MeSH
- Antifungal Agents MeSH
- azoxystrobin MeSH Browser
- DNA-Binding Proteins MeSH
- Phenylacetates MeSH
- Fungal Proteins MeSH
- kresoxim-methyl MeSH Browser
- Methacrylates MeSH
- mucidin MeSH Browser
- Fatty Acids, Unsaturated MeSH
- PDR1 protein, S cerevisiae MeSH Browser
- PDR3 protein, S cerevisiae MeSH Browser
- PDR5 protein, S cerevisiae MeSH Browser
- Fungicides, Industrial MeSH
- Pyrimidines MeSH
- Saccharomyces cerevisiae Proteins MeSH
- Strobilurins MeSH
- Trans-Activators MeSH
- Transcription Factors MeSH
In yeast the resistance to kresoxim-methyl and azoxystrobin, like the resistance to strobilurin A (mucidin) is under the control of both mitochondrial cob gene and the PDR network of nuclear genes involved in multidrug resistance. The mucidin-resistant mucl (G137R) and muc2 (L275S) mutants of Saccharomyces cerevisiae containing point mutations in mtDNA were found to be cross-resistant to kresoxim-methyl and azoxystrobin. Cross-resistance to all three strobilurin fungicides was also observed in yeast transformants containing gain-of-function mutations in the nuclear PDR3 gene. On the other hand, nuclear mutants containing disrupted chromosomal copies of the PDR1 and PDR3 genes or the PDR5 gene alone were hypersensitive to kresoxim-methyl, azoxystrobin and strobilurin A. The frequencies of spontaneous mutants selected for resistance either to kresoxim-methyl, azoxystrobin or strobilurin A were similar and resulted from mutations both in mitochondrial and nuclear genes. The results indicate that resistance to strobilurin fungicides, differing in chemical structure and specific activity, can be caused by the same molecular mechanism involving changes in the structure of apocytochrome b and/or increased efflux of strobilurins from fungal cells.
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