Cross-resistance to strobilurin fungicides in mitochondrial and nuclear mutants of Saccharomyces cerevisiae
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
14533481
DOI
10.1007/bf02931331
Knihovny.cz E-zdroje
- MeSH
- ABC transportéry genetika MeSH
- akryláty farmakologie MeSH
- antifungální látky farmakologie MeSH
- buněčné jádro genetika MeSH
- DNA vazebné proteiny genetika MeSH
- fenylacetáty farmakologie MeSH
- fungální proteiny genetika MeSH
- geny hub MeSH
- methakryláty MeSH
- mikrobiální testy citlivosti MeSH
- mitochondrie genetika MeSH
- mnohočetná fungální léková rezistence genetika MeSH
- mutace MeSH
- nenasycené mastné kyseliny farmakologie MeSH
- průmyslové fungicidy farmakologie MeSH
- pyrimidiny farmakologie MeSH
- Saccharomyces cerevisiae - proteiny genetika MeSH
- Saccharomyces cerevisiae účinky léků genetika MeSH
- strobiluriny MeSH
- transaktivátory genetika MeSH
- transkripční faktory genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- ABC transportéry MeSH
- akryláty MeSH
- antifungální látky MeSH
- azoxystrobin MeSH Prohlížeč
- DNA vazebné proteiny MeSH
- fenylacetáty MeSH
- fungální proteiny MeSH
- kresoxim-methyl MeSH Prohlížeč
- methakryláty MeSH
- mucidin MeSH Prohlížeč
- nenasycené mastné kyseliny MeSH
- PDR1 protein, S cerevisiae MeSH Prohlížeč
- PDR3 protein, S cerevisiae MeSH Prohlížeč
- PDR5 protein, S cerevisiae MeSH Prohlížeč
- průmyslové fungicidy MeSH
- pyrimidiny MeSH
- Saccharomyces cerevisiae - proteiny MeSH
- strobiluriny MeSH
- transaktivátory MeSH
- transkripční faktory MeSH
In yeast the resistance to kresoxim-methyl and azoxystrobin, like the resistance to strobilurin A (mucidin) is under the control of both mitochondrial cob gene and the PDR network of nuclear genes involved in multidrug resistance. The mucidin-resistant mucl (G137R) and muc2 (L275S) mutants of Saccharomyces cerevisiae containing point mutations in mtDNA were found to be cross-resistant to kresoxim-methyl and azoxystrobin. Cross-resistance to all three strobilurin fungicides was also observed in yeast transformants containing gain-of-function mutations in the nuclear PDR3 gene. On the other hand, nuclear mutants containing disrupted chromosomal copies of the PDR1 and PDR3 genes or the PDR5 gene alone were hypersensitive to kresoxim-methyl, azoxystrobin and strobilurin A. The frequencies of spontaneous mutants selected for resistance either to kresoxim-methyl, azoxystrobin or strobilurin A were similar and resulted from mutations both in mitochondrial and nuclear genes. The results indicate that resistance to strobilurin fungicides, differing in chemical structure and specific activity, can be caused by the same molecular mechanism involving changes in the structure of apocytochrome b and/or increased efflux of strobilurins from fungal cells.
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