Dinoroseobacter shibae gen. nov., sp. nov., a new aerobic phototrophic bacterium isolated from dinoflagellates
Language English Country Great Britain, England Media print
Document type Journal Article
PubMed
15879238
DOI
10.1099/ijs.0.63511-0
Knihovny.cz E-resources
- MeSH
- Aerobiosis MeSH
- RNA, Bacterial genetics MeSH
- Bacteriochlorophyll A isolation & purification MeSH
- Sodium Chloride pharmacology MeSH
- Dinoflagellida microbiology MeSH
- DNA, Bacterial chemistry genetics MeSH
- Nitrates metabolism MeSH
- Phenazines MeSH
- Flagella physiology MeSH
- Photosystem II Protein Complex analysis MeSH
- Phylogeny MeSH
- Gentian Violet MeSH
- Genes, rRNA MeSH
- Growth Inhibitors pharmacology MeSH
- Carotenoids isolation & purification MeSH
- Hydrogen-Ion Concentration MeSH
- Fatty Acids analysis MeSH
- Molecular Sequence Data MeSH
- Oxidation-Reduction MeSH
- Movement MeSH
- Rhodobacteraceae classification cytology isolation & purification physiology MeSH
- DNA, Ribosomal chemistry genetics MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Spectrum Analysis MeSH
- Bacterial Typing Techniques MeSH
- Temperature MeSH
- Carbon metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- RNA, Bacterial MeSH
- Bacteriochlorophyll A MeSH
- Sodium Chloride MeSH
- DNA, Bacterial MeSH
- Nitrates MeSH
- Phenazines MeSH
- Photosystem II Protein Complex MeSH
- Gentian Violet MeSH
- Gram's stain MeSH Browser
- Growth Inhibitors MeSH
- Carotenoids MeSH
- Fatty Acids MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal, 16S MeSH
- spheroidenone MeSH Browser
- Carbon MeSH
A novel group of aerobic anoxygenic phototrophic bacteria was isolated from marine dinoflagellates, and two strains were characterized in detail. Cells were Gram-negative cocci or ovoid rods and were motile by means of a single, polarly inserted flagellum. They were obligate aerobes requiring 1-7 % salinity. The optimal pH range for growth was 6.5-9.0 and the temperature optimum was 33 degrees C. The bacteria contained bacteriochlorophyll a and spheroidenone as the only carotenoid. The in vivo absorption spectrum displayed two maxima in the infrared region at 804 and 868 nm. The distinct 804 nm band indicates the presence of light-harvesting system 2. Various organic carbon sources were assimilated, including many carboxylic acids, glucose and glycerol, but not butyrate, ethanol or methanol. Dissimilatory nitrate reduction was found for both strains. The physiological characteristics of the new strains resembled those of Roseobacter denitrificans, but there were differences in the lipid composition. Based on 16S rRNA gene sequence analysis the new strains are relatively distant from other recognized species, with the closest relatives Jannaschia helgolandensis, Ruegeria atlantica and Rhodobacter veldkampii showing 94.1-93.4 % similarity. Similarity to Roseobacter denitrificans was only 92.2 %, in line with numerous other species of the Roseobacter group. Therefore, it is proposed to classify the strains in a new genus and species within the Roseobacter clade, Dinoroseobacter shibae gen. nov., sp. nov. The type strain is DFL 12(T) (=DSM 16493(T)=NCIMB 14021(T)).
References provided by Crossref.org
Horizontal transfers of two types of puf operons among phototrophic members of the Roseobacter clade
GENBANK
AJ534211
