Macroporous hydrogels based on 2-hydroxyethyl methacrylate. Part III. Hydrogels as carriers for immobilization of proteins
Language English Country United States Media print
Document type Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Adsorption MeSH
- Albumins analysis chemistry ultrastructure MeSH
- Avidin analysis chemistry ultrastructure MeSH
- Coated Materials, Biocompatible analysis chemistry MeSH
- Hydrogels analysis chemistry MeSH
- Kinetics MeSH
- Methacrylates analysis chemistry MeSH
- Porosity MeSH
- Surface Properties MeSH
- Materials Testing MeSH
- Protein Binding MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Albumins MeSH
- Avidin MeSH
- Coated Materials, Biocompatible MeSH
- Hydrogels MeSH
- hydroxyethyl methacrylate MeSH Browser
- Methacrylates MeSH
Four series of macroporous hydrogels based on crosslinked copolymers of 2-hydroxyethyl methacrylate (HEMA)-sodium methacrylate (MANa), copolymer HEMA-[2-(methacryloyloxy)ethyl]trimethylammonium chloride (MOETACl), terpolymer HEMA-MANa-MOETACl and on a polyelectrolyte complex were used as carriers for immobilization of proteins, chicken egg white albumin and avidin. The adsorption capacity of the hydrogels for the two proteins, kinetics and pH dependence of albumin adsorption and desorption were studied. The morphology of the hydrogels with and without immobilized albumin was studied by low-vacuum scanning electron microscopy.
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