Yeast fluorescence microscopy
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16118427
DOI
10.1385/1-59259-958-3:085
PII: 1-59259-958-3:085
Knihovny.cz E-resources
- MeSH
- Actins metabolism MeSH
- Cell Wall ultrastructure MeSH
- Chitin metabolism MeSH
- DNA, Fungal metabolism MeSH
- Microscopy, Fluorescence methods MeSH
- Fluorescent Antibody Technique, Indirect MeSH
- DNA, Mitochondrial metabolism MeSH
- Mycology methods MeSH
- Organelles ultrastructure MeSH
- Recombinant Fusion Proteins genetics metabolism MeSH
- Saccharomyces cerevisiae genetics metabolism ultrastructure MeSH
- Green Fluorescent Proteins genetics metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Actins MeSH
- Chitin MeSH
- DNA, Fungal MeSH
- DNA, Mitochondrial MeSH
- Recombinant Fusion Proteins MeSH
- Green Fluorescent Proteins MeSH
Fluorescence microscopy is the essential technique for investigation of the intracellular distribution of macromolecules and various organelles also in yeast cells. In this chapter, detailed practical procedures for fluorescence microscopic observations developed or adopted in our laboratory are described. These include labeling of the cell wall and chitin, F-actin structures, nuclear and mitochondrial DNA, and two different procedures for investigation of yeast cells by immunofluorescence. In addition, our experience with multicolor labeling experiments is introduced and discussed.
References provided by Crossref.org
Formaldehyde fixation is detrimental to actin cables in glucose-depleted S. cerevisiae cells
The fission yeast ortholog of eIF3a subunit is not functional in Saccharomyces cerevisiae
