Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods
Jazyk angličtina Země Německo Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
16138687
DOI
10.1002/jssc.200500070
Knihovny.cz E-zdroje
- MeSH
- chromatografie micelární elektrokinetická kapilární metody MeSH
- elektroforéza kapilární metody MeSH
- epoxidové sloučeniny metabolismus MeSH
- glutathiontransferasa analýza metabolismus MeSH
- játra enzymologie MeSH
- krysa rodu Rattus MeSH
- substrátová specifita MeSH
- techniky in vitro MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- epoxidové sloučeniny MeSH
- glutathiontransferasa MeSH
- styrene oxide MeSH Prohlížeč
A new method has been developed for the monitoring of glutathione S-tranferase (GST) detoxification activity toward styrene oxide (SO). The enzymatic reaction was carried out directly in a thermostatted autosampler vial and the formation of conjugates between glutathione (GSH) and SO was monitored by sequential MEKC runs. The determinations were performed in a 50-microm fused silica capillary using 50 mM SDS in 20 mM phosphate 20 mM tetraborate buffer (pH 8.3) as a background electrolyte; separation voltage 28 kV (positive polarity), temperature of capillary 25 degrees C, and detection at 200 nm. The method is rapid, amenable to automation, and requires only small amounts of samples, which is especially important in the case of GST isoenzyme analyses.
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