Analysis of the molecular variability of Grapevine leafroll-associated virus 1 reveals the presence of two distinct virus groups and their mixed occurrence in grapevines
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Closteroviridae genetics isolation & purification pathogenicity MeSH
- DNA, Viral genetics MeSH
- Phylogeny MeSH
- Genetic Variation MeSH
- Cloning, Molecular MeSH
- Molecular Sequence Data MeSH
- Plant Diseases virology MeSH
- Polymorphism, Restriction Fragment Length MeSH
- HSP70 Heat-Shock Proteins genetics MeSH
- Amino Acid Sequence MeSH
- Base Sequence MeSH
- Sequence Homology, Amino Acid MeSH
- Genes, Viral MeSH
- Viral Proteins genetics MeSH
- Vitis virology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Slovakia MeSH
- Names of Substances
- DNA, Viral MeSH
- HSP70 Heat-Shock Proteins MeSH
- Viral Proteins MeSH
Genetic diversity of eight Grapevine leafroll-associated virus 1 (GLRaV-1) isolates recovered from grapevines in three distinct locations in the Czech Republic and Slovakia was characterised by restriction fragment length polymorphism (RFLP) analysis and by sequencing of cloned 540 bp fragment of the heat shock protein 70 (HSP70) gene. Comparison and phylogenetic analysis of obtained and previously available sequence data revealed the existence of two groups of GLRaV-l isolates, tentatively named A and E (genetic divergence between A and E group reached 13.9%). An RT-PCR detection method followed by simple restriction analysis was developed, showing the potential to differentiate GLRaV-1 isolates of these groups. Interestingly, a mixed infection of two GLRaV-1 groups in the same plant was frequently detected together with a high intra-group variability in some isolates.
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