Genetic diversity of eight Grapevine leafroll-associated virus 1 (GLRaV-1) isolates recovered from grapevines in three distinct locations in the Czech Republic and Slovakia was characterised by restriction fragment length polymorphism (RFLP) analysis and by sequencing of cloned 540 bp fragment of the heat shock protein 70 (HSP70) gene. Comparison and phylogenetic analysis of obtained and previously available sequence data revealed the existence of two groups of GLRaV-l isolates, tentatively named A and E (genetic divergence between A and E group reached 13.9%). An RT-PCR detection method followed by simple restriction analysis was developed, showing the potential to differentiate GLRaV-1 isolates of these groups. Interestingly, a mixed infection of two GLRaV-1 groups in the same plant was frequently detected together with a high intra-group variability in some isolates.

Research Institute of Crop Production Drnovská 507 161 06 Prague 6 Ruzyne Czech Republic

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Virus Res. 2001 Nov 28;80(1-2):109-16 PubMed

J Mol Biol. 1991 Feb 20;217(4):603-10 PubMed

J Gen Virol. 1997 Mar;78 ( Pt 3):535-42 PubMed

Cell. 1998 Feb 6;92(3):351-66 PubMed

Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):14771-6 PubMed

Arch Virol. 2002 Oct;147(10):2039-44 PubMed

J Gen Virol. 1997 Jul;78 ( Pt 7):1511-9 PubMed

J Virol Methods. 2000 Apr;86(1):101-6 PubMed

Proc Natl Acad Sci U S A. 2004 Jan 20;101(3):799-804 PubMed

Arch Virol. 2004 Oct;149(10):1911-29 PubMed

Annu Rev Phytopathol. 2001;39:157-86 PubMed

J Gen Virol. 1994 Jun;75 ( Pt 6):1415-22 PubMed

Bioinformatics. 2001 Dec;17(12):1244-5 PubMed

Trends Biochem Sci. 1998 Oct;23(10):403-5 PubMed

Arch Virol. 2001;146(1):27-40 PubMed

J Gen Virol. 2004 Jul;85(Pt 7):2099-102 PubMed

New Microbiol. 2001 Jan;24(1):99-114 PubMed

J Gen Virol. 2000 Mar;81(Pt 3):605-15 PubMed

J Virol Methods. 2003 May;109(2):217-26 PubMed

Multiple Infections with Viruses of the Family Tymoviridae in Czech Grapevines

Comprehensive Virus Detection Using Next Generation Sequencing in Grapevine Vascular Tissues of Plants Obtained from the Wine Regions of Bohemia and Moravia (Czech Republic)

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GENBANK
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