Expression of beta-catenin is regulated by PI-3 kinase and sodium butyrate in colorectal cancer cells
Jazyk angličtina Země Řecko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
16328013
Knihovny.cz E-zdroje
- MeSH
- adenokarcinom metabolismus MeSH
- alkalická fosfatasa metabolismus MeSH
- androstadieny metabolismus MeSH
- beta-katenin genetika metabolismus MeSH
- butyráty metabolismus MeSH
- fosfatidylinositol-3-kinasy metabolismus MeSH
- fosforylace MeSH
- imunohistochemie MeSH
- inhibitory fosfoinositid-3-kinasy MeSH
- kinasy ribozomálního proteinu S6, 70-kDa metabolismus MeSH
- kinasy ribozomálního proteinu S6 metabolismus MeSH
- kolorektální nádory metabolismus MeSH
- lidé MeSH
- mitogenem aktivované proteinkinasy metabolismus MeSH
- nádorové buněčné linie MeSH
- serin metabolismus MeSH
- signální transdukce fyziologie MeSH
- tyrosin metabolismus MeSH
- wortmannin MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- alkalická fosfatasa MeSH
- androstadieny MeSH
- beta-katenin MeSH
- butyráty MeSH
- inhibitory fosfoinositid-3-kinasy MeSH
- kinasy ribozomálního proteinu S6, 70-kDa MeSH
- kinasy ribozomálního proteinu S6 MeSH
- mitogenem aktivované proteinkinasy MeSH
- serin MeSH
- tyrosin MeSH
- wortmannin MeSH
beta-catenin has a dual function; it is implicated in intercellular junctions and transcriptional co-activation. Here we examined the regulation of the expression and localization of beta-catenin in HT29 colorectal adenocarcinoma cells. Our results showed that inhibition of PI-3 kinase with wortmannin was accompanied by a considerably reduced expression of beta-catenin. This effect was overcome by butyrate and occurred at the protein level, not at the level of mRNA. Moreover, NaBT significantly increased the phosphorylation of the ribosomal protein, S6, known to participate in the translational control of gene expression. This was accompanied by the increased phosphorylation of p70 S6K and MAPKs, the effector proteins that are upstream of protein S6 in the distinct signaling pathways. These facts indicate that different signaling pathways may be involved in the regulation of beta-catenin synthesis. Modulation of beta-catenin expression induced by NaBT appeared to occur at the level of protein translation, suggesting that NaBT may act as a translational regulator.