Use of green fluorescent protein as molecular marker for tagging Bacillus brevis in soil under the control of a novel constitutive promoter F1
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16475504
DOI
10.1007/bf02931426
Knihovny.cz E-resources
- MeSH
- Bacillus genetics physiology MeSH
- Staining and Labeling methods MeSH
- DNA, Bacterial analysis MeSH
- Gene Expression * MeSH
- Fluorescence MeSH
- Genetic Vectors MeSH
- Plant Roots microbiology MeSH
- Plasmids MeSH
- Polymerase Chain Reaction MeSH
- Promoter Regions, Genetic * MeSH
- Soil Microbiology * MeSH
- Genes, Reporter MeSH
- Oryza microbiology MeSH
- Seedlings microbiology MeSH
- Green Fluorescent Proteins analysis genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Green Fluorescent Proteins MeSH
A constitutive expression vector pHY300-Flgfp was constructed to test the function of promoter F1 subcloned from a rice epiphyte Bacillus brevis strain DX01. The DX01 cells harboring plasmid pHY300-F1gfp were detected to produce bright green fluorescence. Subsequently, the gfp-tagged B. brevis strain was released into the soil and its survival was investigated by PCR and the detection of green fluorescence. The spatial location of in situ gfp-tagged bacterial cells on the root surface of rice seedlings was visualized. All these results indicated that green fluorescent protein is an ideal molecular marker for the detection of the activities of promoter F1, and it is also a reliable probe to monitor specific B. brevis bacteria in the environment.
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