Leukemia inhibitory factor gene mutations in the population of infertile women are not restricted to nulligravid patients
Jazyk angličtina Země Irsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
16545901
DOI
10.1016/j.ejogrb.2006.02.008
PII: S0301-2115(06)00107-2
Knihovny.cz E-zdroje
- MeSH
- bodová mutace * MeSH
- dospělí MeSH
- elektroforéza v agarovém gelu metody MeSH
- genetické testování metody MeSH
- gravidita MeSH
- kodon MeSH
- leukemický inhibiční faktor genetika MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- polymerázová řetězová reakce metody MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- těhotenství MeSH
- ženská infertilita genetika MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- kodon MeSH
- leukemický inhibiční faktor MeSH
- LIF protein, human MeSH Prohlížeč
OBJECTIVE: Leukemia inhibitory factor (LIF) is one of the key cytokines in the embryo implantation regulation. We investigated the prevalence of the LIF gene mutations in the population of infertile women that consisted of nulligravid and secondary infertile patients. STUDY DESIGN: We designed a LIF gene mutation screening method that is based on the Temperature Gradient Gel Electrophoresis (TGGE). The population to screen consisted of 176 infertile women including group A of 147 nulligravid women and group B of 29 women with secondary infertility that had a history of either miscarriage or an ectopic pregnancy but no live births. The control population was comprised of 75 healthy fertile subjects. The groups of fertile controls and infertile patients were compared for statistically significant differences using the t-test. RESULTS: Six potentially functional LIF gene mutations, the G to A transitions at the position 3400 leading to the valin to methionin exchange at codon 64 (V64M) in the AB loop region of the LIF protein, were detected. All of the six positive women were infertile. Four of them were nulligravid and two of them had history of spontaneous conception followed by early miscarriage. No positive TGGE samples were identified in the control group, which means that the frequency of functionally relevant mutations of the LIF gene in infertile women is significantly enhanced in comparison with controls (P<0.05, t-test). CONCLUSION: The results suggest that the LIF gene mutations affect fertility. Even though they occur infrequently, their impact on molecular events during early phases of pregnancy should be further established.
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