Evaluation of mitochondrial membrane potential using a computerized device with a tetraphenylphosphonium-selective electrode
Language English Country United States Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
16643832
DOI
10.1016/j.ab.2006.03.032
PII: S0003-2697(06)00212-0
Knihovny.cz E-resources
- MeSH
- Electrophysiology instrumentation methods MeSH
- Indicators and Reagents chemistry MeSH
- Ion-Selective Electrodes * MeSH
- Mitochondria, Liver metabolism MeSH
- Rats MeSH
- Humans MeSH
- Membrane Potentials physiology MeSH
- Mitochondrial Membranes metabolism MeSH
- Tumor Cells, Cultured MeSH
- Onium Compounds chemistry MeSH
- Organophosphorus Compounds chemistry MeSH
- Computer Peripherals MeSH
- Computer Systems MeSH
- Rats, Wistar MeSH
- Reproducibility of Results MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Humans MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Indicators and Reagents MeSH
- Onium Compounds MeSH
- Organophosphorus Compounds MeSH
- tetraphenylphosphonium MeSH Browser
Mitochondrial membrane potential (Deltapsi(m)) plays important roles in the normal function of cells and in pathobiochemical situations. The application of ion-selective electrodes for the measurement of Deltapsi(m) is important for studying normal biological reactions and pathways and mitochondrial diseases. We constructed and optimized a computerized device for real-time monitoring of the Deltapsi(m), which included modification of tetraphenylphosphonium (TPP(+))-selective membrane that improved reproducibility of the TPP(+)-selective electrode. Application of MATLAB software increased the sensitivity of the system. We tested our improved device for membrane potential measurements of isolated mitochondria (in absolute scale of millivolts). In addition, we assessed relative changes of Deltapsi(m) (as changes in TPP(+) concentration) of digitonin-permeabilized cells (hepatocytes, control transmitochondrial cybrids, HeLa G and BSC-40) after addition of substrates, inhibitors, and uncoupler of respiratory chain. Our system can be successfully used for studies of many aspects of the regulation of mitochondrial bioenergetics and as a diagnostic tool for mitochondrial oxidative phosphorylation disorders.
References provided by Crossref.org
Pleiotropic Effects of Biguanides on Mitochondrial Reactive Oxygen Species Production
Cyanide inhibition and pyruvate-induced recovery of cytochrome c oxidase
