-
Je něco špatně v tomto záznamu ?
Genome-wide screening of cytogenetic abnormalities in multiple myeloma patients using array-CGH technique: a Czech multicenter experience
J. Smetana, J. Frohlich, R. Zaoralova, V. Vallova, H. Greslikova, R. Kupska, P. Nemec, A. Mikulasova, M. Almasi, L. Pour, Z. Adam, V. Sandecka, L. Zahradová, R. Hajek, P. Kuglik,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu klinické zkoušky, časopisecké články, multicentrická studie, práce podpořená grantem
Grantová podpora
NT13492
MZ0
CEP - Centrální evidence projektů
NLK
Free Medical Journals
od 2013
Hindawi Publishing Open Access
od 2001-01-01
PubMed Central
od 2013
Europe PubMed Central
od 2013
ProQuest Central
od 2013
Open Access Digital Library
od 2001-01-01
Open Access Digital Library
od 2012-12-04
Open Access Digital Library
od 2013-01-01
CINAHL Plus with Full Text (EBSCOhost)
od 2013-01-01
Medline Complete (EBSCOhost)
od 2013-01-01
Health & Medicine (ProQuest)
od 2013
ROAD: Directory of Open Access Scholarly Resources
od 2013
PubMed
24987674
DOI
10.1155/2014/209670
Knihovny.cz E-zdroje
- MeSH
- celogenomová asociační studie * MeSH
- chromozomální aberace * MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mnohočetný myelom diagnóza genetika MeSH
- nádorové proteiny genetika MeSH
- prognóza MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- srovnávací genomová hybridizace metody MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- klinické zkoušky MeSH
- multicentrická studie MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
Characteristic recurrent copy number aberrations (CNAs) play a key role in multiple myeloma (MM) pathogenesis and have important prognostic significance for MM patients. Array-based comparative genomic hybridization (aCGH) provides a powerful tool for genome-wide classification of CNAs and thus should be implemented into MM routine diagnostics. We demonstrate the possibility of effective utilization of oligonucleotide-based aCGH in 91 MM patients. Chromosomal aberrations associated with effect on the prognosis of MM were initially evaluated by I-FISH and were found in 93.4% (85/91). Incidence of hyperdiploidy was 49.5% (45/91); del(13)(q14) was detected in 57.1% (52/91); gain(1)(q21) occurred in 58.2% (53/91); del(17)(p13) was observed in 15.4% (14/91); and t(4;14)(p16;q32) was found in 18.6% (16/86). Genome-wide screening using Agilent 44K aCGH microarrays revealed copy number alterations in 100% (91/91). Most common deletions were found at 13q (58.9%), 1p (39.6%), and 8p (31.1%), whereas gain of whole 1q was the most often duplicated region (50.6%). Furthermore, frequent homozygous deletions of genes playing important role in myeloma biology such as TRAF3, BIRC1/BIRC2, RB1, or CDKN2C were observed. Taken together, we demonstrated the utilization of aCGH technique in clinical diagnostics as powerful tool for identification of unbalanced genomic abnormalities with prognostic significance for MM patients.
Department of Clinical Hematology University Hospital Brno Bohunice 62500 Brno Czech Republic
Department of Internal Hematooncology University Hospital Brno Bohunice 62500 Brno Czech Republic
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc15014169
- 003
- CZ-PrNML
- 005
- 20191011102001.0
- 007
- ta
- 008
- 150420s2014 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1155/2014/209670 $2 doi
- 035 __
- $a (PubMed)24987674
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Smetana, Jan $u Department of Experimental Biology, Faculty of Science, Masaryk University Brno, Bohunice, 62500 Brno, Czech Republic ; Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University Brno, Bohunice, 62500 Brno, Czech Republic. $7 xx0128923
- 245 10
- $a Genome-wide screening of cytogenetic abnormalities in multiple myeloma patients using array-CGH technique: a Czech multicenter experience / $c J. Smetana, J. Frohlich, R. Zaoralova, V. Vallova, H. Greslikova, R. Kupska, P. Nemec, A. Mikulasova, M. Almasi, L. Pour, Z. Adam, V. Sandecka, L. Zahradová, R. Hajek, P. Kuglik,
- 520 9_
- $a Characteristic recurrent copy number aberrations (CNAs) play a key role in multiple myeloma (MM) pathogenesis and have important prognostic significance for MM patients. Array-based comparative genomic hybridization (aCGH) provides a powerful tool for genome-wide classification of CNAs and thus should be implemented into MM routine diagnostics. We demonstrate the possibility of effective utilization of oligonucleotide-based aCGH in 91 MM patients. Chromosomal aberrations associated with effect on the prognosis of MM were initially evaluated by I-FISH and were found in 93.4% (85/91). Incidence of hyperdiploidy was 49.5% (45/91); del(13)(q14) was detected in 57.1% (52/91); gain(1)(q21) occurred in 58.2% (53/91); del(17)(p13) was observed in 15.4% (14/91); and t(4;14)(p16;q32) was found in 18.6% (16/86). Genome-wide screening using Agilent 44K aCGH microarrays revealed copy number alterations in 100% (91/91). Most common deletions were found at 13q (58.9%), 1p (39.6%), and 8p (31.1%), whereas gain of whole 1q was the most often duplicated region (50.6%). Furthermore, frequent homozygous deletions of genes playing important role in myeloma biology such as TRAF3, BIRC1/BIRC2, RB1, or CDKN2C were observed. Taken together, we demonstrated the utilization of aCGH technique in clinical diagnostics as powerful tool for identification of unbalanced genomic abnormalities with prognostic significance for MM patients.
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a senioři $7 D000368
- 650 _2
- $a senioři nad 80 let $7 D000369
- 650 12
- $a chromozomální aberace $7 D002869
- 650 _2
- $a srovnávací genomová hybridizace $x metody $7 D055028
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 12
- $a celogenomová asociační studie $7 D055106
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a lidé středního věku $7 D008875
- 650 _2
- $a mnohočetný myelom $x diagnóza $x genetika $7 D009101
- 650 _2
- $a nádorové proteiny $x genetika $7 D009363
- 650 _2
- $a prognóza $7 D011379
- 651 _2
- $a Česká republika $7 D018153
- 655 _2
- $a klinické zkoušky $7 D016430
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a multicentrická studie $7 D016448
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Fröhlich, Jan. $u Department of Experimental Biology, Faculty of Science, Masaryk University Brno, Bohunice, 62500 Brno, Czech Republic ; Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University Brno, Bohunice, 62500 Brno, Czech Republic. $7 xx0240724
- 700 1_
- $a Zaoralová, Romana $u Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University Brno, Bohunice, 62500 Brno, Czech Republic. $7 xx0126082
- 700 1_
- $a Vallová, Vladimíra, $u Department of Experimental Biology, Faculty of Science, Masaryk University Brno, Bohunice, 62500 Brno, Czech Republic. $d 1979- $7 mub2011669456
- 700 1_
- $a Grešliková, Henrieta $u Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University Brno, Bohunice, 62500 Brno, Czech Republic. $7 xx0122004
- 700 1_
- $a Kupská, Renata $u Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University Brno, Bohunice, 62500 Brno, Czech Republic. $7 xx0128684
- 700 1_
- $a Němec, Pavel $u Department of Experimental Biology, Faculty of Science, Masaryk University Brno, Bohunice, 62500 Brno, Czech Republic ; Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University Brno, Bohunice, 62500 Brno, Czech Republic. $7 xx0162732
- 700 1_
- $a Mikulášová, Aneta $u Department of Experimental Biology, Faculty of Science, Masaryk University Brno, Bohunice, 62500 Brno, Czech Republic ; Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University Brno, Bohunice, 62500 Brno, Czech Republic. $7 _AN050306
- 700 1_
- $a Almasi, Martina $u Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University Brno, Bohunice, 62500 Brno, Czech Republic ; Department of Internal Hematooncology, University Hospital Brno, Bohunice, 62500 Brno, Czech Republic. $7 gn_A_00004597
- 700 1_
- $a Pour, Luděk $u Department of Clinical Hematology, University Hospital Brno, Bohunice, 62500 Brno, Czech Republic. $7 xx0102556
- 700 1_
- $a Adam, Zdeněk $u Department of Clinical Hematology, University Hospital Brno, Bohunice, 62500 Brno, Czech Republic. $7 jn19981000018
- 700 1_
- $a Sandecká, Viera $u Department of Clinical Hematology, University Hospital Brno, Bohunice, 62500 Brno, Czech Republic. $7 xx0129325
- 700 1_
- $a Szeligová, Lenka $u Faculty of Medicine, University of Ostrava and University Hospital Ostrava, Poruba, 70852 Ostrava, Czech Republic. $7 xx0098951
- 700 1_
- $a Hájek, Roman, $u Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University Brno, Bohunice, 62500 Brno, Czech Republic ; Department of Internal Hematooncology, University Hospital Brno, Bohunice, 62500 Brno, Czech Republic ; Faculty of Medicine, University of Ostrava and University Hospital Ostrava, Poruba, 70852 Ostrava, Czech Republic. $d 1964- $7 nlk20000083645
- 700 1_
- $a Kuglík, Petr, $u Department of Experimental Biology, Faculty of Science, Masaryk University Brno, Bohunice, 62500 Brno, Czech Republic. $d 1957- $7 ola2003204793
- 773 0_
- $w MED00182164 $t BioMed research international $x 2314-6141 $g Roč. 2014, č. - (2014), s. 209670
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/24987674 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20150420 $b ABA008
- 991 __
- $a 20191011102421 $b ABA008
- 999 __
- $a ok $b bmc $g 1071750 $s 897047
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2014 $b 2014 $c - $d 209670 $i 2314-6141 $m BioMed research international $n Biomed Res Int $x MED00182164
- GRA __
- $a NT13492 $p MZ0
- LZP __
- $a Pubmed-20150420