Primer R108 performs best in the RAPD strain typing of three Aspergillus species frequently isolated from patients
Language English Country United States Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
16821724
DOI
10.1007/bf02932169
Knihovny.cz E-resources
- MeSH
- Aspergillus flavus classification MeSH
- Aspergillus fumigatus classification MeSH
- Aspergillus niger classification MeSH
- Aspergillosis diagnosis microbiology MeSH
- DNA Fingerprinting methods MeSH
- DNA Primers MeSH
- Humans MeSH
- Mycological Typing Techniques methods MeSH
- Reproducibility of Results MeSH
- Random Amplified Polymorphic DNA Technique methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA Primers MeSH
We evaluated the suitability of various primers for the RAPD (random amplified polymorphic DNA) accurate species identification and strain typing of Aspergillus clinical isolates. Five primers described previously were tested for their discriminatory power in three Aspergillus species (A. fumigatus, A. niger agg. and A. flavus - 23 clinical isolates and 2 reference strains). Clustering of RAPD fingerprints corresponded well with the identification based on morphological features. All isolates were resolved as different strains using the primer R108 and the RAPD protocol optimized for a Robocycler thermal cycler. RAPD with the primer R108 thus can be considered to be a valuable, simple and powerful tool for identification and strain delineation of Aspergillus spp.
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