Amplified oligonucleotide sensing in microliter volumes containing copper ions by solution streaming
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16841945
DOI
10.1021/ac052227o
Knihovny.cz E-resources
- MeSH
- Adenine chemistry MeSH
- Argon chemistry MeSH
- DNA Probes chemistry genetics MeSH
- Guanine chemistry MeSH
- Ions chemistry MeSH
- Copper chemistry MeSH
- Microscopy, Atomic Force MeSH
- Oligonucleotides chemistry genetics MeSH
- Solutions MeSH
- Base Sequence MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adenine MeSH
- Argon MeSH
- DNA Probes MeSH
- Guanine MeSH
- Ions MeSH
- Copper MeSH
- Oligonucleotides MeSH
- Solutions MeSH
We present a simple, cost-effective design for amplifying oligodeoxynucleotide (ODN) sensing, in microliter ODN volumes containing copper ions, by solution streaming (bubbling). The inert gas streaming (bubbling) at a constant pressure of 0.04 bar drives the motion of a 30-microL ODN droplet containing a three-electrode circuit (inverted drop microcell), and in the presence of copper ions offers an approximately 50-times improvement in the detection of ODN samples. The detection of ODNs at the carbon paste electrode is based on the enhancement of the oxidation peaks of purine bases (adenine and guanine) by the anodic stripping of the electrochemically accumulated complex of Cu(I) with purine base residues of acid hydrolyzed ODN samples (Cu(I)-ahODN complex). We used the proposed method for (i) the determination of the percentage content of adenine and guanine units within analyzed ODN samples at subnanomolar concentrations (related to monomer content) and (ii) the detection of the (TTC)n triplet expansion using magnetic DNA hybridization with reporter probes containing guanine units (the TTC trinucleotide repeat expansion is associated with serious hereditary diseases, including Friedreich ataxia).
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