Ergosterol treatment leads to the expression of a specific set of defence-related genes in tobacco
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- cholesterol farmakologie MeSH
- DNA primery MeSH
- DNA rostlinná genetika MeSH
- ergosterol farmakologie MeSH
- genetická transkripce účinky léků MeSH
- regulace genové exprese u rostlin účinky léků MeSH
- RNA rostlin genetika MeSH
- rostlinné proteiny genetika MeSH
- sekvence nukleotidů MeSH
- tabák účinky léků genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cholesterol MeSH
- DNA primery MeSH
- DNA rostlinná MeSH
- ergosterol MeSH
- RNA rostlin MeSH
- rostlinné proteiny MeSH
Ergosterol is the main sterol of most fungi. Production of reactive oxygen species after the treatment of tobacco and tomato cells by nano-molar concentrations of ergosterol was previously observed as well as the activation of some stress activated mitogen-activated protein kinases on alfalfa cells. In this paper, the expression of some defence-related genes after the ergosterol treatment of tobacco Nicotiana tabacum plants is reported. The gene expression of pathogenesis related proteins of families PR1, PR3, PR5 and proteinase inhibitors of class I and II together with enzymes participating in the defence response, such as phenylalanine-ammonia lyase and sesquiterpene cyclase, were monitored by RT-qPCR. In addition, the concentrations of salicylic acid, an important signalling molecule, increased in time due to the enzyme activation. On the other hand, ergosterol did not provoke tissue necrosis and the possible cross-talk between the signalling pathways of salicylate and jasmonate was observed. Collected data shows that ergosterol is able to activate the expression of a number of defence genes and could increase resistance against pathogens.
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