Changes of acetylcholinesterase activity in different rat brain areas following intoxication with nerve agents: biochemical and histochemical study
Jazyk angličtina Země Irsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
17145052
DOI
10.1016/j.cbi.2006.10.006
PII: S0009-2797(06)00306-1
Knihovny.cz E-zdroje
- MeSH
- acetylcholinesterasa metabolismus MeSH
- apoptóza účinky léků MeSH
- bazální ganglia účinky léků enzymologie patologie MeSH
- čelní lalok účinky léků enzymologie patologie MeSH
- chemické bojové látky toxicita MeSH
- cholinesterasové inhibitory toxicita MeSH
- krysa rodu Rattus MeSH
- potkani Wistar MeSH
- sarin aplikace a dávkování toxicita MeSH
- soman aplikace a dávkování toxicita MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- acetylcholinesterasa MeSH
- chemické bojové látky MeSH
- cholinesterasové inhibitory MeSH
- sarin MeSH
- soman MeSH
Acetylcholinesterase activity in defined brain regions was determined using biochemical and histochemical methods 30 min after treating rats with sarin, soman or VX (0.5 x LD(50)). Enzyme inhibition was high in the pontomedullar area and frontal cortex, but was low in the basal ganglia. Histochemical and biochemical results correlated well. Determination of the activity in defined brain structures was a more sensitive parameter than determination in whole brain homogenate where the activity was a "mean" of the activities in different structures. The pontomedullar area controls respiration, so that the special sensitivity of acetylcholinesterase to inhibition by nerve agents in this area is important for understanding the mechanism of death caused by nerve agents. Thus, acetylcholinesterase activity is the main parameter investigated in studies searching for target sites following nerve agent poisoning.
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