Changes of acetylcholinesterase activity in different rat brain areas following intoxication with nerve agents: biochemical and histochemical study
Language English Country Ireland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17145052
DOI
10.1016/j.cbi.2006.10.006
PII: S0009-2797(06)00306-1
Knihovny.cz E-resources
- MeSH
- Acetylcholinesterase metabolism MeSH
- Apoptosis drug effects MeSH
- Basal Ganglia drug effects enzymology pathology MeSH
- Frontal Lobe drug effects enzymology pathology MeSH
- Chemical Warfare Agents toxicity MeSH
- Cholinesterase Inhibitors toxicity MeSH
- Rats MeSH
- Rats, Wistar MeSH
- Sarin administration & dosage toxicity MeSH
- Soman administration & dosage toxicity MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Acetylcholinesterase MeSH
- Chemical Warfare Agents MeSH
- Cholinesterase Inhibitors MeSH
- Sarin MeSH
- Soman MeSH
Acetylcholinesterase activity in defined brain regions was determined using biochemical and histochemical methods 30 min after treating rats with sarin, soman or VX (0.5 x LD(50)). Enzyme inhibition was high in the pontomedullar area and frontal cortex, but was low in the basal ganglia. Histochemical and biochemical results correlated well. Determination of the activity in defined brain structures was a more sensitive parameter than determination in whole brain homogenate where the activity was a "mean" of the activities in different structures. The pontomedullar area controls respiration, so that the special sensitivity of acetylcholinesterase to inhibition by nerve agents in this area is important for understanding the mechanism of death caused by nerve agents. Thus, acetylcholinesterase activity is the main parameter investigated in studies searching for target sites following nerve agent poisoning.
References provided by Crossref.org
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