Tick-borne encephalitis virus in Clethrionomys glareolus in the Czech Republic
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Arvicolinae virology MeSH
- Hantavirus Infections epidemiology transmission veterinary MeSH
- Orthohantavirus isolation & purification MeSH
- Encephalitis, Tick-Borne epidemiology transmission veterinary MeSH
- Kidney virology MeSH
- Rodent Diseases epidemiology transmission MeSH
- Lung virology MeSH
- Polymerase Chain Reaction methods MeSH
- RNA, Viral chemistry genetics MeSH
- Sequence Homology MeSH
- Spleen virology MeSH
- Encephalitis Viruses, Tick-Borne isolation & purification MeSH
- Disease Reservoirs veterinary MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Names of Substances
- RNA, Viral MeSH
A total of 474 specimens from 157 rodents caught at the military training area of Boletice in the south of the Czech Republic from May to November 1999 were screened for TBEV by nested PCR. TBEV-specific RNA was amplified from lung, kidney, and spleen derived from one Clethrionomys glareolus in the first RT-PCR round. Sequence analysis revealed a 100% identity to the TBEV strain Neudoerfl. TBEV presence in the sample was confirmed by mouse brain passage of backup samples and cell culture. The results support the observation that hantaviruses and TBEV transmission can occur sympatrically in the same rodent population.
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