Differentiation-specific association of HP1alpha and HP1beta with chromocentres is correlated with clustering of TIF1beta at these sites
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- azacytidin analogy a deriváty farmakologie MeSH
- buněčná diferenciace účinky léků fyziologie MeSH
- buněčné jádro metabolismus MeSH
- centromera metabolismus MeSH
- chromozomální proteiny, nehistonové genetika metabolismus MeSH
- decitabin MeSH
- homolog proteinu s chromoboxem 5 MeSH
- imunohistochemie MeSH
- inhibitory enzymů farmakologie MeSH
- inhibitory histondeacetylas MeSH
- jaderné proteiny metabolismus MeSH
- konfokální mikroskopie MeSH
- kyseliny hydroxamové farmakologie MeSH
- metylace DNA účinky léků MeSH
- nádorové buněčné linie MeSH
- podjednotky proteinů genetika metabolismus MeSH
- rekombinantní fúzní proteiny genetika metabolismus MeSH
- signální transdukce účinky léků MeSH
- transkripční faktory metabolismus MeSH
- western blotting MeSH
- zelené fluorescenční proteiny genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- azacytidin MeSH
- chromozomální proteiny, nehistonové MeSH
- decitabin MeSH
- homolog proteinu s chromoboxem 5 MeSH
- inhibitory enzymů MeSH
- inhibitory histondeacetylas MeSH
- jaderné proteiny MeSH
- kyseliny hydroxamové MeSH
- podjednotky proteinů MeSH
- rekombinantní fúzní proteiny MeSH
- transcriptional intermediary factor 1 MeSH Prohlížeč
- transkripční faktory MeSH
- trichostatin A MeSH Prohlížeč
- zelené fluorescenční proteiny MeSH
Mammalian heterochromatin protein 1 (HP1alpha, HP1beta, HP1gamma subtypes) and transcriptional intermediary factor TIF1beta play an important role in the regulation of chromatin structure and function. Here, we investigated the nuclear arrangement of these proteins during differentiation of embryonal carcinoma P19 cells into primitive endoderm and into the neural pathway. Additionally, the differentiation potential of trichostatin A (TSA) and 5-deoxyazacytidine (5-dAzaC) was studied. In 70% of the cells from the neural pathway and in 20% of TSA-stimulated cells, HP1alpha and HP1beta co-localized and associated with chromocentres (clusters of centromeres), which correlated with clustering of TIF1beta at these heterochromatic regions. The cell types that we studied were also characterized by a pronounced focal distribution of HP1gamma. The above-mentioned nuclear patterns of HP1 and TIF1beta proteins were completely different from the nuclear patterns observed in the remaining cell types investigated, in which HP1alpha was associated with chromocentres while HP1beta and HP1gamma were largely localized in distinct nuclear regions. Moreover, a dispersed nuclear distribution of TIF1beta was observed. Our findings showed that the nuclear arrangement of HP1 subtypes and TIF1beta is differentiation specific, and seems to be more important than changes in the levels of these proteins, which were relatively stable during all the induced differentiation processes.
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