Isolation and characterization of human CXCR4-positive pancreatic cells
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17328838
PII: file/6144/fb2007a0003.pdf
Knihovny.cz E-resources
- MeSH
- Cell Differentiation MeSH
- C-Peptide metabolism MeSH
- Adult MeSH
- Fluorescent Antibody Technique MeSH
- Glucagon metabolism MeSH
- Insulin metabolism MeSH
- Cells, Cultured MeSH
- Middle Aged MeSH
- Humans MeSH
- RNA, Messenger genetics metabolism MeSH
- Nestin MeSH
- Pancreas cytology MeSH
- Intermediate Filament Proteins metabolism MeSH
- Nerve Tissue Proteins metabolism MeSH
- Leukemia Inhibitory Factor Receptor alpha Subunit metabolism MeSH
- Receptors, CXCR4 genetics metabolism MeSH
- Gene Expression Regulation MeSH
- Aged MeSH
- Cell Separation * MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Aged MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- C-Peptide MeSH
- Glucagon MeSH
- Insulin MeSH
- LIFR protein, human MeSH Browser
- RNA, Messenger MeSH
- NES protein, human MeSH Browser
- Nestin MeSH
- Intermediate Filament Proteins MeSH
- Nerve Tissue Proteins MeSH
- Leukemia Inhibitory Factor Receptor alpha Subunit MeSH
- Receptors, CXCR4 MeSH
The existence of an adult PSC that may be used in the treatment of diabetes is still a matter of scientific debate as conclusive evidence of such a stem cell in the adult pancreas has not yet been presented. The main reason why putative PSC has not yet been identified is the lack of specific markers that may be used to isolate and purify them. In order to increase the list of potential PSC markers we have focused on the human pancreatic cells that express cell surface receptor CXCR4, a marker of stem cells derived from different adult tissues. Here we report that CXCR4-positive pancreatic cells express markers of pancreatic endocrine progenitors (neurogenin-3, nestin) and markers of pluripotent stem cells (Oct-4, Nanog, ABCG2, CD133, CD117). Upon in vitro differentiation, these cells form ILCC and produce key islet hormones including insulin. Based on our results, we assume that CXCR4 marks pancreatic endocrine progenitors and in combination with other cell surface markers may be used in the attempt to identify and isolate PSC.
